A seed coat bedding assay to genetically explore in vitro how the endosperm controls seed germination in Arabidopsis thaliana

Lee, Keun Pyo; Lopez Molina, Luis

doi:10.3791/50732

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Title		A seed coat bedding assay to genetically explore in vitro how the endosperm controls seed germination in Arabidopsis thaliana
Authors		Lee, Keun Pyo Lopez Molina, Luis
Published in		Journal of visualized experiments. 2013, vol. 81, e50732
Abstract		The Arabidopsis endosperm consists of a single cell layer surrounding the mature embryo and playing an essential role to prevent the germination of dormant seeds or that of nondormant seeds irradiated by a far red (FR) light pulse. In order to further gain insight into the molecular genetic mechanisms underlying the germination repressive activity exerted by the endosperm, a "seed coat bedding" assay (SCBA) was devised. The SCBA is a dissection procedure physically separating seed coats and embryos from seeds, which allows monitoring the growth of embryos on an underlying layer of seed coats. Remarkably, the SCBA reconstitutes the germination repressive activities of the seed coat in the context of seed dormancy and FR-dependent control of seed germination. Since the SCBA allows the combinatorial use of dormant, nondormant and genetically modified seed coat and embryonic materials, the genetic pathways controlling germination and specifically operating in the endosperm and embryo can be dissected. Here we detail the procedure to assemble a SCBA.
Identifiers		DOI: 10.3791/50732 PMID: 24300527
Full text		Supplemental data (542 Kb) - Free access Article (Published version) (2.7 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département des sciences végétales
Research group		Groupe Lopez-Molina
Citation (ISO format)		LEE, Keun Pyo, LOPEZ MOLINA, Luis. A seed coat bedding assay to genetically explore in vitro how the endosperm controls seed germination in Arabidopsis thaliana. In: Journal of visualized experiments, 2013, vol. 81, p. e50732. doi: 10.3791/50732 https://archive-ouverte.unige.ch/unige:43510