Isolation of genetic material from Arabidopsis seeds

Piskurewicz, Urszula; Lopez Molina, Luis

doi:10.1007/978-1-61779-231-1_10

Scientific article

English

Isolation of genetic material from Arabidopsis seeds

ContributorsPiskurewicz, Urszula; Lopez Molina, Luis

Published inMethods in molecular biology, vol. 773, p. 151-164

Publication date2011

Abstract

Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Keywords

Arabidopsis/genetics
DNA, Plant/genetics/isolation & purification
Plant Proteins/genetics/isolation & purification
RNA, Plant/genetics/isolation & purification
Seeds/chemistry/genetics

Affiliation entities

Faculté des sciences / Section de biologie / Département des sciences végétales

Research groups

Groupe Lopez-Molina

Citation (ISO format)

PISKUREWICZ, Urszula, LOPEZ MOLINA, Luis. Isolation of genetic material from Arabidopsis seeds. In: Methods in molecular biology, 2011, vol. 773, p. 151–164. doi: 10.1007/978-1-61779-231-1_10

Article (Published version)

Identifiers

PID : unige:43503
DOI : 10.1007/978-1-61779-231-1_10
PMID : 21898255

Journal ISSN1064-3745

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Isolation of genetic material from Arabidopsis seeds

Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Technical informations