UNIGE document Professional Article
previous document  unige:43389  next document
add to browser collection

Identification of the tetraspanin CD82 as a new barrier to xenotransplantation

Saleh, Soad M
Parhar, Ranjit S
Al-Hejailan, Reem S
Bakheet, Razan H
Khaleel, Hala S
Khalak, Hanif G
Halees, Anason S
Zaidi, Marya Z
show hidden authors show all authors [1 - 15]
Published in The Journal of immunology. 2013, vol. 191, no. 5, p. 2796-805
Abstract Significant immunological obstacles are to be negotiated before xenotransplantation becomes a clinical reality. An initial rejection of transplanted vascularized xenograft is attributed to Galα1,3Galβ1,4GlcNAc-R (Galα1,3-Gal)-dependent and -independent mechanisms. Hitherto, no receptor molecule has been identified that could account for Galα1,3-Gal-independent rejection. In this study, we identify the tetraspanin CD82 as a receptor molecule for the Galα1,3-Gal-independent mechanism. We demonstrate that, in contrast to human undifferentiated myeloid cell lines, differentiated cell lines are capable of recognizing xenogeneic porcine aortic endothelial cells in a calcium-dependent manner. Transcriptome-wide analysis to identify the differentially expressed transcripts in these cells revealed that the most likely candidate of the Galα1,3-Gal-independent recognition moiety is the tetraspanin CD82. Abs to CD82 inhibited the calcium response and the subsequent activation invoked by xenogeneic encounter. Our data identify CD82 on innate immune cells as a major "xenogenicity sensor" and open new avenues of intervention to making xenotransplantation a clinical reality.
Keywords AnimalsAntigens, CD82/immunologyBlotting, WesternEndothelial Cells/immunologyFlow CytometryFluorescent Antibody TechniqueGraft Rejection/immunologyHumansMicroscopy, ConfocalReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSwineTransplantation, Heterologous/immunology
PMID: 23872050
Full text
Article (Published version) (1.7 MB) - public document Free access
Research group Laboratoire d'immunologie translationnelle (856)
(ISO format)
SALEH, Soad M et al. Identification of the tetraspanin CD82 as a new barrier to xenotransplantation. In: The Journal of immunology, 2013, vol. 191, n° 5, p. 2796-805. doi: 10.4049/jimmunol.1300601 https://archive-ouverte.unige.ch/unige:43389

541 hits



Deposited on : 2014-12-15

Export document
Format :
Citation style :