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Title

A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana

Authors
Chua, N H
Published in Plant and Cell Physiology. 2000, vol. 41, no. 5, p. 541-547
Abstract We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper.
Keywords Abscisic Acid/pharmacologyAmino Acid SequenceArabidopsis/drug effects/genetics/physiologyChromosome MappingConserved SequenceGenetic Complementation TestLeucine ZippersMolecular Sequence DataPlant Growth Regulators/pharmacologyPlant Proteins/geneticsPolymerase Chain ReactionSequence AlignmentSequence Homology, Amino AcidSignal Transduction/drug effectsTranscription Factors/genetics
Identifiers
PMID: 10929936
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Article (Published version) (428 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Research group Groupe Lopez-Molina
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LOPEZ MOLINA, Luis, CHUA, N H. A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana. In: Plant and Cell Physiology, 2000, vol. 41, n° 5, p. 541-547. https://archive-ouverte.unige.ch/unige:43381

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Deposited on : 2014-12-15

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