UNIGE document Scientific Article
previous document  unige:43359  next document
add to browser collection
Title

Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs

Authors
Published in Proceedings of the National Academy of Sciences. 1997, vol. 94, no. 13, p. 6831-6836
Abstract We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotin- and restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of liver cDNA from a mouse strain expressing the neomycin resistance gene demonstrated that this procedure is capable of identifying species present in one population but absent from another. Furthermore, experiments to identify genes with circadian expression patterns in mouse liver demonstrated that SABRE is capable of detecting even modest 2- to 10-fold differences in accumulation of moderately rare mRNA species, representing as little as 0.03% of total mRNA. These experiments identified the gene encoding coumarin 7-hydroxylase as displaying circadian expression in mouse liver.
Keywords AnimalsAryl Hydrocarbon HydroxylasesBiotinCircadian RhythmCytochrome P-450 Enzyme System/geneticsDNA, Complementary/analysis/geneticsHumansLiver/enzymologyMiceMixed Function Oxygenases/geneticsNucleic Acid HybridizationPolymerase Chain Reaction/methodsRNA, Messenger/analysis
Identifiers
PMID: 9192651
Full text
Article (Published version) (347 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.pnas.org/content/94/13/6831.short
Structures
Citation
(ISO format)
LAVERY, Daniel et al. Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs. In: Proceedings of the National Academy of Sciences, 1997, vol. 94, n° 13, p. 6831-6836. https://archive-ouverte.unige.ch/unige:43359

205 hits

0 download

Update

Deposited on : 2014-12-15

Export document
Format :
Citation style :