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Scientific article
English

Effect of chloride ions on the kinetic parameters of the potato tuber and mung bean pyrophosphate-dependent phosphofructokinase

Contributors

Degli Agosti, Robert; Van Praag, Esther; Greppin, Hubert

Published in

Biochemistry international, vol. 26, p. 707-713

Publication date

1992

Abstract

The affinity constant Ka of PPi-PFK for Fru 2,6-P2 is equal to 1.56 nM for the potato enzyme and to 6.67 nM for that of the mung bean in the absence of chloride ions. These results are notably lower than the currently reported 5.5 nM and 30, 50 nM respectively. It is shown that the chloride ion is a competitive inhibitor of Fru 2,6-P2 for both enzymes. The inhibition constant Ki is equal to 15.6 mM for potato PPi-PFK up to 40 mM chloride. For the mung bean enzyme, the Ki is 19.0 mM up to 30 mM chloride. No effects are detected on the Michaelis­Menten constants Km of the substrates Fru-6-P and PPi up to 40 mM chloride. Other halide ions are also found to inhibit the potato PPi-PFK: bromide is competitive like chloride, whereas fluoride and iodide have a mixed inhibition towards Fru 2,6-

UNIGE affiliation entities

Citation (ISO format)

DEGLI AGOSTI, Robert, VAN PRAAG, Esther, GREPPIN, Hubert. Effect of chloride ions on the kinetic parameters of the potato tuber and mung bean pyrophosphate-dependent phosphofructokinase. In: Biochemistry international, 1992, vol. 26, p. 707–713.

Main files (1)

Article (Published version)

Identifiers

  • PID : unige:42836

Journal ISSN

0158-5231
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Technical informations

Creation

05/12/2014 10:47:00

First validation

05/12/2014 10:47:00

Update

14/03/2023 22:23:02

Status update

14/03/2023 22:23:02

Last indexation

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