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Scientific article
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Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model

Published inPloS one, vol. 8, no. 7, e67847
Publication date2013
Abstract

Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii) five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days) than K. pneumoniae reference strain (LT50: 4.3 days) (p<0.01). However, the worldwide spread KPC-2 positive K. pneumoniae ST258 strains and reference strains containing plasmids extracted from K. pneumoniae ST258 strains had a higher virulence than KPC-2 strains belonging to other ST types (LT50: 5 days vs. 6 days, p<0.01). The increased virulence observed in cured strains confirmed this trend. The bla KPC-2 gene itself was not associated to increased virulence.

Keywords
  • Animals
  • Anti-Bacterial Agents/pharmacology
  • Bacterial Proteins/genetics/metabolism
  • Caenorhabditis elegans/growth & development/microbiology
  • Host-Pathogen Interactions
  • Klebsiella pneumoniae/enzymology/pathogenicity/physiology
  • Microbial Sensitivity Tests
  • Microbial Viability/drug effects
  • Plasmids/genetics/metabolism
  • Species Specificity
  • Time Factors
  • Virulence/genetics
  • Beta-Lactamases/genetics/metabolism
Citation (ISO format)
LAVIGNE, Jean-Philippe et al. Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model. In: PloS one, 2013, vol. 8, n° 7, p. e67847. doi: 10.1371/journal.pone.0067847
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Article (Published version)
Identifiers
ISSN of the journal1932-6203
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