UNIGE document Scientific Article
previous document  unige:42548  next document
add to browser collection
Title

Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model
Authors
Lavigne, Jean-Philippe
Cuzon, Gaelle
Combescure, Christophe
Bourg, Gisèle
Sotto, Albert
Nordmann, Patrice
Published in PLOS ONE. 2013, vol. 8, no. 7, e67847
Abstract Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii) five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days) than K. pneumoniae reference strain (LT50: 4.3 days) (p<0.01). However, the worldwide spread KPC-2 positive K. pneumoniae ST258 strains and reference strains containing plasmids extracted from K. pneumoniae ST258 strains had a higher virulence than KPC-2 strains belonging to other ST types (LT50: 5 days vs. 6 days, p<0.01). The increased virulence observed in cured strains confirmed this trend. The bla KPC-2 gene itself was not associated to increased virulence.
Keywords Animals — Anti-Bacterial Agents/pharmacology — Bacterial Proteins/genetics/metabolism — Caenorhabditis elegans/growth & development/microbiology — Host-Pathogen Interactions — Klebsiella pneumoniae/enzymology/pathogenicity/physiology — Microbial Sensitivity Tests — Microbial Viability/drug effects — Plasmids/genetics/metabolism — Species Specificity — Time Factors — Virulence/genetics — Beta-Lactamases/genetics/metabolism
Identifiers
PMID: 23844109
Full text
Article (Published version) (110 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires
Research group Epidémiologie clinique (115)
Citation
(ISO format) 		LAVIGNE, Jean-Philippe et al. Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model. In: PLOS ONE, 2013, vol. 8, n° 7, p. e67847. doi: 10.1371/journal.pone.0067847 https://archive-ouverte.unige.ch/unige:42548

456 hits

209 downloads

Contact an author

Update request

Update

Deposited on : 2014-12-05

Export document
Format :
Citation style :