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Scientific article
English

Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation

Published inRCM. Rapid communications in mass spectrometry, vol. 27, no. 1, p. 200-206
Publication date2013
Abstract

The aim of the work was to develop and validate a method for the quantification of vitamin D metabolites in serum using ultra-high-pressure liquid chromatography coupled to mass spectrometry (LC/MS), and to validate a high-resolution mass spectrometry (LC/HRMS) approach against a tandem mass spectrometry (LC/MS/MS) approach using a large clinical sample set.

Keywords
  • 25-Hydroxyvitamin D 2/blood
  • Calcifediol/blood
  • Chromatography, High Pressure Liquid/methods
  • Humans
  • Linear Models
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry/methods
Citation (ISO format)
BRUCE, Stephen J et al. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation. In: RCM. Rapid communications in mass spectrometry, 2013, vol. 27, n° 1, p. 200–206. doi: 10.1002/rcm.6439
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Article (Published version)
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ISSN of the journal0951-4198
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Technical informations

Creation10/14/2014 2:24:00 PM
First validation10/14/2014 2:24:00 PM
Update time03/14/2023 10:20:10 PM
Status update03/14/2023 10:20:10 PM
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