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Title

Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation

Authors
Bruce, Stephen J
Rochat, Bertrand
Pesse, Benoît
Boulat, Olivier
Henry, Hugues
Published in Rapid Communications in Mass Spectrometry. 2013, vol. 27, no. 1, p. 200-6
Abstract The aim of the work was to develop and validate a method for the quantification of vitamin D metabolites in serum using ultra-high-pressure liquid chromatography coupled to mass spectrometry (LC/MS), and to validate a high-resolution mass spectrometry (LC/HRMS) approach against a tandem mass spectrometry (LC/MS/MS) approach using a large clinical sample set.
Keywords 25-Hydroxyvitamin D 2/bloodCalcifediol/bloodChromatography, High Pressure Liquid/methodsHumansLinear ModelsReproducibility of ResultsSensitivity and SpecificityTandem Mass Spectrometry/methods
Identifiers
PMID: 23239334
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Research group Groupe Jean-Michel Gaspoz (23)
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BRUCE, Stephen J et al. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation. In: Rapid Communications in Mass Spectrometry, 2013, vol. 27, n° 1, p. 200-6. https://archive-ouverte.unige.ch/unige:42437

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Deposited on : 2014-12-02

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