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Title

Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation
Authors
Bruce, Stephen J
Rochat, Bertrand
Beguin, Alexandre
Pesse, Benoît
Guessous, Idris
Boulat, Olivier
Henry, Hugues
Published in RCM. Rapid communications in mass spectrometry. 2013, vol. 27, no. 1, p. 200-6
Abstract The aim of the work was to develop and validate a method for the quantification of vitamin D metabolites in serum using ultra-high-pressure liquid chromatography coupled to mass spectrometry (LC/MS), and to validate a high-resolution mass spectrometry (LC/HRMS) approach against a tandem mass spectrometry (LC/MS/MS) approach using a large clinical sample set.
Keywords 25-Hydroxyvitamin D 2/blood — Calcifediol/blood — Chromatography, High Pressure Liquid/methods — Humans — Linear Models — Reproducibility of Results — Sensitivity and Specificity — Tandem Mass Spectrometry/methods
Identifiers
PMID: 23239334
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Structures
Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine de premier recours
Research group Groupe Jean-Michel Gaspoz (23)
Citation
(ISO format) 		BRUCE, Stephen J et al. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation. In: RCM. Rapid communications in mass spectrometry, 2013, vol. 27, n° 1, p. 200-6. doi: 10.1002/rcm.6439 https://archive-ouverte.unige.ch/unige:42437

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Deposited on : 2014-12-02

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