Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation

Bruce, Stephen J; Rochat, Bertrand; Beguin, Alexandre; Pesse, Benoît; Guessous, Idris; Boulat, Olivier; Henry, Hugues

doi:10.1002/rcm.6439

Scientific article

English

Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation

ContributorsBruce, Stephen J; Rochat, Bertrand; Beguin, Alexandre; Pesse, Benoît; Guessous, Idris; Boulat, Olivier; Henry, Hugues

Published inRCM. Rapid communications in mass spectrometry, vol. 27, no. 1, p. 200-206

Publication date2013

Abstract

The aim of the work was to develop and validate a method for the quantification of vitamin D metabolites in serum using ultra-high-pressure liquid chromatography coupled to mass spectrometry (LC/MS), and to validate a high-resolution mass spectrometry (LC/HRMS) approach against a tandem mass spectrometry (LC/MS/MS) approach using a large clinical sample set.

Keywords

25-Hydroxyvitamin D 2/blood
Calcifediol/blood
Chromatography, High Pressure Liquid/methods
Humans
Linear Models
Reproducibility of Results
Sensitivity and Specificity
Tandem Mass Spectrometry/methods

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine de premier recours

Research groups

Groupe Jean-Michel Gaspoz (23)

Citation (ISO format)

BRUCE, Stephen J et al. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation. In: RCM. Rapid communications in mass spectrometry, 2013, vol. 27, n° 1, p. 200–206. doi: 10.1002/rcm.6439

Article (Published version)

Identifiers

PID : unige:42437
DOI : 10.1002/rcm.6439
PMID : 23239334

Journal ISSN0951-4198

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Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation

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