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Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation |
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Published in | RCM. Rapid communications in mass spectrometry. 2013, vol. 27, no. 1, p. 200-6 | |
Abstract | The aim of the work was to develop and validate a method for the quantification of vitamin D metabolites in serum using ultra-high-pressure liquid chromatography coupled to mass spectrometry (LC/MS), and to validate a high-resolution mass spectrometry (LC/HRMS) approach against a tandem mass spectrometry (LC/MS/MS) approach using a large clinical sample set. | |
Keywords | 25-Hydroxyvitamin D 2/blood — Calcifediol/blood — Chromatography, High Pressure Liquid/methods — Humans — Linear Models — Reproducibility of Results — Sensitivity and Specificity — Tandem Mass Spectrometry/methods | |
Identifiers | DOI: 10.1002/rcm.6439 PMID: 23239334 | |
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Research group | Groupe Jean-Michel Gaspoz (23) | |
Citation (ISO format) | BRUCE, Stephen J et al. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry--a method comparison and validation. In: RCM. Rapid communications in mass spectrometry, 2013, vol. 27, n° 1, p. 200-6. doi: 10.1002/rcm.6439 https://archive-ouverte.unige.ch/unige:42437 |