UNIGE document Scientific Article
previous document  unige:41741  next document
add to browser collection

Secretome profiling of differentiated neural mes-c-myc A1 cell line endowed with stem cell properties

Colucci-D'Amato, Luca
Reccia, Mafalda Giovanna
Volpicelli, Floriana
Parente, Augusto
Chambery, Angela
Published in Biochimica et Biophysica Acta. 2013, vol. 1834, no. 11, p. 2385-95
Abstract Neural stem cell proliferation and differentiation play a crucial role in the formation and wiring of neuronal connections forming neuronal circuits. During neural tissues development, a large diversity of neuronal phenotypes is produced from neural precursor cells. In recent years, the cellular and molecular mechanisms by which specific types of neurons are generated have been explored with the aim to elucidate the complex events leading to the generation of different phenotypes via distinctive developmental programs that control self-renewal, differentiation, and plasticity. The extracellular environment is thought to provide instructive influences that actively induce the production of specific neuronal phenotypes. In this work, the secretome profiling of differentiated neural mes-c-myc A1 (A1) cell line endowed with stem cell properties was analyzed by applying a shotgun LC-MS/MS approach. The results provide a list of secreted molecules with potential relevance for the functional and biological features characterizing the A1 neuronal phenotype. Proteins involved in biological processes closely related to nervous system development including neurites growth, differentiation of neurons and axonogenesis were identified. Among them, proteins belonging to extracellular matrix and cell-adhesion complexes as well as soluble factors with well established neurotrophic properties were detected. The presented work provides the basis to clarify the complex extracellular protein networks implicated in neuronal differentiation and in the acquisition of the neuronal phenotype. This article is part of a Special Issue entitled: An Updated Secretome.
Keywords AnimalsCell LineChromatography, Liquid/methodsExtracellular Matrix/chemistry/metabolismMiceNeural Stem Cells/cytology/metabolismNeurogenesisProtein Interaction MapsProteome/analysis/metabolism/secretionProteomics/methodsSecretory PathwayTandem Mass Spectrometry/methods
PMID: 23246712
Full text
Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group Chimie et protéomique clinique (270)
(ISO format)
SEVERINO, Valeria et al. Secretome profiling of differentiated neural mes-c-myc A1 cell line endowed with stem cell properties. In: Biochimica et Biophysica Acta, 2013, vol. 1834, n° 11, p. 2385-95. https://archive-ouverte.unige.ch/unige:41741

209 hits

0 download


Deposited on : 2014-11-12

Export document
Format :
Citation style :