en
Scientific article
English

Positive association of DRB1 04 and DRB1 15 alleles with Fya immunization in a Southern European population

Published inTransfusion, vol. 49, no. 11, p. 2412-2417
Publication date2009
Abstract

BACKGROUND: Anti-Fy(a) has been implicated in hemolytic transfusion reactions. However, not all Fy(a-) patients develop anti-Fy(a) after transfusion with 1 unit of blood [Fy(a+)]. This study was designed to identify HLA-DRB1 alleles associated with a predisposition to Fy(a) immunization after blood transfusion. STUDY DESIGN AND METHODS: To identify HLA-DRB1 alleles prone to immunization after blood transfusion or pregnancy, HLA-DRB1 genotyping using polymerase chain reaction with sequence-specific oligonucleotide nonradioactive probe/sequence-specific priming methods was performed on blood samples from 67 immunized patients and 200 unrelated controls from the same southern European population in a case-control retrospective study. RESULTS: Ninety-six percent of patients with anti-Fy(a) had at least one HLA-DRB1*04 or HLA-DRB1*15 allele compared to 34% of controls (p(c) < 0.001). Furthermore HLA-DRB1*04 and HLA-DRB1*1501 frequencies were significantly increased in Fy(a)-immunized patients (35% vs. 12%, p(c) < 0.001; and 30% vs. 19%, p(c) < 0.001, respectively). Among HLA-DRB1*04 allelic subtypes, DRB1*0401 and DRB1*0403 alleles were more strongly correlated with Fy(a) immunization (51% vs. 24% and 19% vs. 9%; p(c) < 0.001, respectively). CONCLUSIONS: This study indicated that HLA-DRB1*04 and DRB1*1501 are overrepresented in Fy(a)-immunized patients. The correlation between these alleles and Fy(a) immunization could be due to a particular presentation of the Fy(a) peptide in HLA-DRB1 molecules.

Keywords
  • Human genetics
  • HLA
  • Immunization
Citation (ISO format)
PICARD, Christophe et al. Positive association of DRB1 04 and DRB1 15 alleles with Fya immunization in a Southern European population. In: Transfusion, 2009, vol. 49, n° 11, p. 2412–2417. doi: 10.1111/j.1537-2995.2009.02369.x
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ISSN of the journal0041-1132
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