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Title

Factorial design optimization and in vivo feasibility of poly(epsilon-caprolactone)-micro- and nanofiber-based small diameter vascular grafts

Authors
Mandracchia, D.
Published in Journal of Biomedical Materials Research. A. 2009, vol. 89, no. 4, p. 865-875
Abstract Because of the severe increase of mortality by cardiovascular diseases, there has been rising interest among the tissue-engineering community for small-sized blood vessel substitutes. Here we present small diameter vascular grafts made of slow degradable poly(epsilon-caprolactone) nanofibers obtained by electrospinning. The process was optimized by a factorial design approach that led to reproducible grafts with inner diameters of 2 and 4 mm, respectively. Fiber sizes, graft morphology, and the resulting tensile stress and tensile strain values were studied as a function of various parameters in order to obtain optimal vascular grafts for implantation after gamma-sterilization. The influence of polymer concentration, solvent, needle-collector distance, applied voltage, flow rate, and spinning time has been studied. Consequently, an optimized vascular graft was implanted as an abdominal aortic substitute in nine rats for a feasibility study. Results are given following up a 12-week implantation period showing good patency, endothelization, and cell ingrowth.
Keywords AngiographyAnimalsBlood Vessel ProsthesisBlood Vessels/cytology/transplantation/ultrastructureFeasibility StudiesImplants, ExperimentalNanostructures/chemistryPilot ProjectsPolyesters/pharmacologyRatsSolventsSurface Properties/drug effectsTensile Strength/drug effectsTissue Engineering/methods
Identifiers
PMID: 18465817
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NOTTELET, Benjamin et al. Factorial design optimization and in vivo feasibility of poly(epsilon-caprolactone)-micro- and nanofiber-based small diameter vascular grafts. In: Journal of Biomedical Materials Research. A, 2009, vol. 89, n° 4, p. 865-875. https://archive-ouverte.unige.ch/unige:4102

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Deposited on : 2009-11-24

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