Mechanisms of local invasion in enteroendocrine tumors: Identification of novel candidate cytoskeleton-associated proteins in an experimental mouse model by a proteomic approach and validation in human tumors

Couderc, Christophe; Bollard, Julien; Coute, Yohann; Massoma, Patrick; Poncet, Gilles; Lepinasse, Florian; Hervieu, Valérie; Gadot, Nicolas; Sanchez, Jean-Charles; Scoazec, Jean-Yves; Diaz, Jean-Jacques; Roche, Colette

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Title		Mechanisms of local invasion in enteroendocrine tumors: Identification of novel candidate cytoskeleton-associated proteins in an experimental mouse model by a proteomic approach and validation in human tumors
Authors		Couderc, Christophe Bollard, Julien Coute, Yohann Massoma, Patrick Poncet, Gilles Lepinasse, Florian Hervieu, Valérie Gadot, Nicolas Sanchez, Jean-Charles Scoazec, Jean-Yves Diaz, Jean-Jacques Roche, Colette show all authors [1 - 12]
Published in		Molecular and Cellular Endocrinology. 2015, vol. 399, p. 154-163
Abstract		Small-intestinal neuroendocrine tumors (SI-NETs) are defined as locally invasive only after extension to the muscularis propria. To gain further insight into the molecular mechanisms, we applied a proteomic approach to an orthotopic xenograft model to identify candidate proteins evaluable in human SI-NETs. After grafting STC-1 neuroendocrine tumor cells on the caecum of nude mice, comparative proteomic studies were performed between the pre-invasive and the invasive stages, respectively 2 and 8 weeks after grafting. We identified 24 proteins displaying at least a 1.5-fold differential expression between 2 and 8 week-stages. Most were cytoskeleton-associated proteins, among which five showed decreasing expression levels (CRMP2, TCP1ε, TPM2, vimentin, desmin) and two increasing expression levels (14-3-3γ, CK8). Changes for CRMP2, TCP1ε, TPM2 and 14-3-3γ were confirmed in experimental tumors and in a series of 28 human SI-NETs. In conclusion, our results underline the relevance of proteomics to identify novel biomarkers of tissue invasion.
Identifiers		DOI: 10.1016/j.mce.2014.09.006 PMID: 25224486
Full text		Article (Published version) (2.9 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group		Groupe de Protéomique biomédicale (635)
Citation (ISO format)		COUDERC, Christophe et al. Mechanisms of local invasion in enteroendocrine tumors: Identification of novel candidate cytoskeleton-associated proteins in an experimental mouse model by a proteomic approach and validation in human tumors. In: Molecular and Cellular Endocrinology, 2015, vol. 399, p. 154-163. https://archive-ouverte.unige.ch/unige:40832