UNIGE document Scientific Article
previous document  unige:40832  next document
add to browser collection
Title

Mechanisms of local invasion in enteroendocrine tumors: Identification of novel candidate cytoskeleton-associated proteins in an experimental mouse model by a proteomic approach and validation in human tumors

Authors
Bollard, Julien
Massoma, Patrick
Poncet, Gilles
Lepinasse, Florian
Hervieu, Valérie
Gadot, Nicolas
show hidden authors show all authors [1 - 12]
Published in Molecular and Cellular Endocrinology. 2015, vol. 399, p. 154-163
Abstract Small-intestinal neuroendocrine tumors (SI-NETs) are defined as locally invasive only after extension to the muscularis propria. To gain further insight into the molecular mechanisms, we applied a proteomic approach to an orthotopic xenograft model to identify candidate proteins evaluable in human SI-NETs. After grafting STC-1 neuroendocrine tumor cells on the caecum of nude mice, comparative proteomic studies were performed between the pre-invasive and the invasive stages, respectively 2 and 8 weeks after grafting. We identified 24 proteins displaying at least a 1.5-fold differential expression between 2 and 8 week-stages. Most were cytoskeleton-associated proteins, among which five showed decreasing expression levels (CRMP2, TCP1ε, TPM2, vimentin, desmin) and two increasing expression levels (14-3-3γ, CK8). Changes for CRMP2, TCP1ε, TPM2 and 14-3-3γ were confirmed in experimental tumors and in a series of 28 human SI-NETs. In conclusion, our results underline the relevance of proteomics to identify novel biomarkers of tissue invasion.
Identifiers
PMID: 25224486
Full text
Article (Published version) (2.9 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe de Protéomique biomédicale (635)
Citation
(ISO format)
COUDERC, Christophe et al. Mechanisms of local invasion in enteroendocrine tumors: Identification of novel candidate cytoskeleton-associated proteins in an experimental mouse model by a proteomic approach and validation in human tumors. In: Molecular and Cellular Endocrinology, 2015, vol. 399, p. 154-163. https://archive-ouverte.unige.ch/unige:40832

265 hits

3 downloads

Update

Deposited on : 2014-10-13

Export document
Format :
Citation style :