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Membrane expression of Annexin‐V on human monocytes: Implication in 'silent phagocytosis' of apoptotic cells

ContributorsPadayachy, Laura
Master program titleMaster thesis in Biology
Defense date2014
Abstract

Phenotypic analysis demonstrated the expression of high levels of Annexin-V (AV) on the outer membrane of monocytes (MO) which was down-regulated by stimulation with lipopolysaccharide (LPS) or IL-4 and GM-CSF. In contrast, IFNγ stimulation increased the expression of AV on MO. Functional phagocytosis assays revealed that MO phagocytose phosphatidylserine (PS) expressing apoptotic cells (AC) in an AV-dependent manner. Both, blocking AV/PS interaction using a monoclonal anti-AV antibody and recombinant AV significantly diminished the phagocytosis of syngeneic AC. Interestingly, phagocytosis of allogeneic AC was selectively increased by blocking AV on MO, whereas blocking of PS on allogeneic AC led to a decrease. Taken together, our data show for the first time a distinct function of surface expressed AV in PS recognition and a subsequent ‘silent phagocytosis' by MO. These findings improve the understanding of AC clearance and might be of interest for the development of new anti-inflammatory cellular therapies.

eng
Keywords
  • Monocytes
  • Annexin-V
  • Apoptotic cells
  • Phagocytosis
  • Clearance
Citation (ISO format)
PADAYACHY, Laura. Membrane expression of Annexin‐V on human monocytes: Implication in “silent phagocytosis” of apoptotic cells. 2014.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:40619
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Technical informations

Creation09/30/2014 2:36:00 PM
First validation09/30/2014 2:36:00 PM
Update time03/14/2023 9:48:40 PM
Status update03/14/2023 9:48:40 PM
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