Dextran sulfate acts as an endothelial cell protectant and inhibits human complement and natural killer cell-mediated cytotoxicity against porcine cells

Laumonier, Thomas; Walpen, Alexander J; Maurus, Christine F; Mohacsi, Paul J; Matozan, Katja M; Korchagina, Elena Y; Bovin, Nicolai V; Vanhove, Bernard; Seebach, Jorg Dieter; Rieben, Robert

doi:10.1097/01.TP.0000078898.28399.0A

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Title		Dextran sulfate acts as an endothelial cell protectant and inhibits human complement and natural killer cell-mediated cytotoxicity against porcine cells
Authors		Laumonier, Thomas Walpen, Alexander J Maurus, Christine F Mohacsi, Paul J Matozan, Katja M Korchagina, Elena Y Bovin, Nicolai V Vanhove, Bernard Seebach, Jorg Dieter Rieben, Robert show all authors [1 - 10]
Published in		Transplantation. 2003, vol. 76, no. 5, p. 838-43
Abstract		The innate immune system, including complement and natural killer (NK) cells, plays a critical role in activation and damage of endothelial cells (ECs) during xenograft rejection. The semisynthetic proteoglycan analog dextran sulfate (DXS, molecular weight 5,000) is known to inhibit the complement and coagulation cascades. We hypothesized that DXS may act as an "EC-protectant" preventing complement and NK lysis by functionally replacing heparan sulfate proteoglycans that are shed from the EC surface on activation of the endothelium.
Keywords		Animals — Anticoagulants/metabolism/pharmacology — Aorta/cytology — Blood Proteins/pharmacology — Cells, Cultured — Complement Activation/drug effects — Complement Inactivator Proteins/pharmacology — Dextran Sulfate/metabolism/pharmacology — Dose-Response Relationship, Drug — Endothelium, Vascular/cytology/drug effects/immunology — Graft Rejection/drug therapy/immunology — Heparin Lyase/pharmacology — Humans — Killer Cells, Natural/drug effects/immunology — Swine — Transplantation, Heterologous/immunology
Identifiers		DOI: 10.1097/01.TP.0000078898.28399.0A PMID: 14501864
Full text		Article (Published version) (252 Kb) - Limited access to UNIGE
Citation (ISO format)		LAUMONIER, Thomas et al. Dextran sulfate acts as an endothelial cell protectant and inhibits human complement and natural killer cell-mediated cytotoxicity against porcine cells. In: Transplantation, 2003, vol. 76, n° 5, p. 838-43. doi: 10.1097/01.TP.0000078898.28399.0A https://archive-ouverte.unige.ch/unige:40448