On-line and At-line Liquid Chromatography Nuclear Magnetic Resonance and Related Micro-Nuclear Magnetic Resonance Methods in Natural Product Analysis

Bohni, Nadine; Ferreira Queiroz, Emerson; Wolfender, Jean-Luc

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Title		On-line and At-line Liquid Chromatography Nuclear Magnetic Resonance and Related Micro-Nuclear Magnetic Resonance Methods in Natural Product Analysis
Authors		Bohni, Nadine Ferreira Queiroz, Emerson Wolfender, Jean-Luc
Published in		K. Hostettmann, S. Chen, A. Marston, H. Stuppner. Encyclopedia of Analytical Chemistry. Chichester, UK: Wiley. 2014
Abstract		Nuclear magnetic resonance spectroscopy (NMR) is essential for the de novo structure identification of natural products. This technique can be directly or indirectly hyphenated to high performance liquid chromatography (HPLC) leading to efficient LC-NMR platforms. NMR is, however, a very insensitive detection method compared to mass spectrometry and several strategies for its efficient hyphenation with HPLC have been developed. This includes on-line LC-NMR, SPE-NMR, or other at-line approaches involving microflow probes or microtubes in combination with cryoprobes. All of these various technologies and their potentials and limitations in the field of natural product research are described. Recent applications involving other complementary detection methods such as LC-MS or biological HPLC profiling illustrate its utilization.
Keywords		LC-NMR — SPE-NMR — Microflow NMR — Micro-NMR — Cryo-NMR — Natural products — Phytochemical analysis — Dereplication — Metabolite profiling
Identifiers		DOI: 10.1002/9780470027318.a9916
Full text		Book chapter (Published version) (1.7 MB) - Limited access to UNIGE
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Phytochimie et produits naturels bioactifs
Projects		FNS: CRSII3_127187 FNS: 205320-124667/1
Citation (ISO format)		BOHNI, Nadine, FERREIRA QUEIROZ, Emerson, WOLFENDER, Jean-Luc. On-line and At-line Liquid Chromatography Nuclear Magnetic Resonance and Related Micro-Nuclear Magnetic Resonance Methods in Natural Product Analysis. In: K. Hostettmann, S. Chen, A. Marston, H. Stuppner (Ed.). Encyclopedia of Analytical Chemistry. Chichester, UK : Wiley, 2014. https://archive-ouverte.unige.ch/unige:39953