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prdl-a, a gene marker for hydra apical differentiation related to triploblastic paired-like head-specific genes

Kreger, S
Holstein, T
Published in Development. 1998, vol. 125, no. 9, p. 1637-1645
Abstract Two homeobox genes, prdl-a and prdl-b, which were isolated from a Hydra vulgaris cDNA library, encode paired-like class homeodomains highly related to that of the aristaless-related genes. In adult polyps, prdl-b is a marker for synchronously dividing nematoblasts while prdl-a displays an expression restricted to the the nerve cell lineage of the head region. During budding and apical regeneration, an early and transient prdl-a expression was observed in endodermal cells of the stump at a time when the head organizer is established. When apical regeneration was delayed upon concomittant budding, prdl-a expression was found to be altered in the stump. Furthermore, a specific anti-prdl-a protein immunoserum revealed that prdl-a was overexpressed in adult polyps of the Chlorohydra viridissima multiheaded mutant, with an expression domain extending below the tentacle ring towards the body column. Accordingly, prdl-a DNA-binding activity was enhanced in nuclear extracts from this mutant. These results suggest that prdl-a responds to apical forming signals and might thus be involved in apical specification. When a marine hydrozoan (Podocorynae carnea) was used, the anti-prdl-a antibody showed cross-reactivity with cells located around the oral region, indicating that prdl-a function is shared by other cnidaria. The ancestral role for prdl-a-related genes in the molecular definition of the head (or oral-surrounding region) is discussed.
Keywords Amino Acid SequenceAnimalsBase SequenceBiological MarkersDNA/metabolismDNA, Complementary/geneticsEctoderm/chemistryGene Expression Regulation, DevelopmentalGenes, Homeobox/geneticsHead/abnormalities/physiologyHomeodomain Proteins/analysis/geneticsHydra/genetics/growth & developmentMolecular Sequence DataRNA, Messenger/analysisRegenerationTranscription Factors
PMID: 9521902
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Research group Groupe Galliot
Swiss National Science Foundation: 3100-039801.93; 3100-49665-96
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GAUCHAT-FEISS, Dominique Madeleine et al. prdl-a, a gene marker for hydra apical differentiation related to triploblastic paired-like head-specific genes. In: Development, 1998, vol. 125, n° 9, p. 1637-1645. https://archive-ouverte.unige.ch/unige:39943

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Deposited on : 2014-09-03

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