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Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy

Tirado-Gonzalez, I.
Freitag, N.
Barrientos, G.
Shaikly, V.
Nagaeva, O.
Strand, M.
Kjellberg, L.
Klapp, B. F.
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Published in Molecular Human Reproduction. 2013, vol. 19, no. 1, p. 43-53
Abstract Galectin-1 (gal-1) is expressed at the feto-maternal interface and plays a role in regulating the maternal immune response against placental alloantigens, contributing to pregnancy maintenance. Both decidua and placenta contribute to gal-1 expression and may be important for the maternal immune regulation. The expression of gal-1 within the placenta is considered relevant to cell-adhesion and invasion of trophoblasts, but the role of gal-1 in the immune evasion machinery exhibited by trophoblast cells remains to be elucidated. In this study, we analyzed gal-1 expression in preimplantation human embryos and first-trimester decidua-placenta specimens and serum gal-1 levels to investigate the physiological role played by this lectin during pregnancy. The effect on human leukocyte antigen G (HLA-G) expression in response to stimulation or silencing of gal-1 was also determined in the human invasive, proliferative extravillous cytotrophoblast 65 (HIPEC65) cell line. Compared with normal pregnant women, circulating gal-1 levels were significantly decreased in patients who subsequently suffered a miscarriage. Human embryos undergoing preimplantation development expressed gal-1 on the trophectoderm and inner cell mass. Furthermore, our in vitro experiments showed that exogenous gal-1 positively regulated the membrane-bound HLA-G isoforms (HLA-G1 and G2) in HIPEC65 cells, whereas endogenous gal-1 also induced expression of the soluble isoforms (HLA-G5 and -G6). Our results suggest that gal-1 plays a key role in pregnancy maternal immune regulation by modulating HLA-G expression on trophoblast cells. Circulating gal-1 levels could serve as a predictive factor for pregnancy success in early human gestation.
Keywords Galectin-1TrophoblastPredictive factor
PMID: 23002109
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Research group Laboratoire de biologie des tumeurs gynécologiques et du développement (927)
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TIRADO-GONZALEZ, I. et al. Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy. In: Molecular Human Reproduction, 2013, vol. 19, n° 1, p. 43-53. https://archive-ouverte.unige.ch/unige:39901

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Deposited on : 2014-09-02

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