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Atomic resolution insight into host cell recognition by Toxoplasma gondii

Blumenschein, Tharin M A.
Childs, Robert A.
Saouros, Savvas
Carpenter, Elisabeth P.
Campanero-Rhodes, Maria A.
Simpson, Peter
Chai, Wengang
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Published in EMBO Journal. 2007, vol. 26, no. 11, p. 2808-20
Abstract The obligate intracellular parasite Toxoplasma gondii, a member of the phylum Apicomplexa that includes Plasmodium spp., is one of the most widespread parasites and the causative agent of toxoplasmosis. Micronemal proteins (MICs) are released onto the parasite surface just before invasion of host cells and play important roles in host cell recognition, attachment and penetration. Here, we report the atomic structure for a key MIC, TgMIC1, and reveal a novel cell-binding motif called the microneme adhesive repeat (MAR). Using glycoarray analyses, we identified a novel interaction with sialylated oligosaccharides that resolves several prevailing misconceptions concerning TgMIC1. Structural studies of various complexes between TgMIC1 and sialylated oligosaccharides provide high-resolution insights into the recognition of sialylated oligosaccharides by a parasite surface protein. We observe that MAR domains exist in tandem repeats, which provide a highly specialized structure for glycan discrimination. Our work uncovers new features of parasite-receptor interactions at the early stages of host cell invasion, which will assist the design of new therapeutic strategies.
Keywords Amino Acid SequenceAnimalsCell Adhesion Molecules/genetics/metabolismCrystallizationDNA Primers/geneticsHost-Parasite InteractionsModels, MolecularMolecular Sequence DataNeuraminidaseNuclear Magnetic Resonance, BiomolecularProtein Array AnalysisProtein Structure, Tertiary/geneticsProtozoan Proteins/genetics/metabolismToxoplasma/genetics
PMID: 17491595
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Research group Biologie d'un parasite intracellulaire obligatoire (773)
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BLUMENSCHEIN, Tharin M A. et al. Atomic resolution insight into host cell recognition by Toxoplasma gondii. In: EMBO journal, 2007, vol. 26, n° 11, p. 2808-20. doi: 10.1038/sj.emboj.7601704 https://archive-ouverte.unige.ch/unige:3972

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Deposited on : 2009-11-05

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