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Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii

Kwok, Lai-Yu
Sheiner, Lilach
Schepers, Rebecca
Published in PLOS Pathogens. 2007, vol. 3, no. 8, p. e115
Abstract Toxoplasma gondii is an aerobic protozoan parasite that possesses mitochondrial antioxidant enzymes to safely dispose of oxygen radicals generated by cellular respiration and metabolism. As with most Apicomplexans, it also harbors a chloroplast-like organelle, the apicoplast, which hosts various biosynthetic pathways and requires antioxidant protection. Most apicoplast-resident proteins are encoded in the nuclear genome and are targeted to the organelle via a bipartite N-terminal targeting sequence. We show here that two antioxidant enzymes-a superoxide dismutase (TgSOD2) and a thioredoxin-dependent peroxidase (TgTPX1/2)-and an aconitase are dually targeted to both the apicoplast and the mitochondrion of T. gondii. In the case of TgSOD2, our results indicate that a single gene product is bimodally targeted due to an inconspicuous variation within the putative signal peptide of the organellar protein, which significantly alters its subcellular localization. Dual organellar targeting of proteins might occur frequently in Apicomplexans to serve important biological functions such as antioxidant protection and carbon metabolism.
Keywords Aconitate Hydratase/metabolismAmino Acid SequenceAnimalsDNA Primers/chemistryDNA, Protozoan/analysisMitochondria/enzymologyMolecular Sequence DataPeroxidases/metabolismSequence AlignmentSuperoxide Dismutase/metabolismToxoplasma/cytology/enzymology/genetics
PMID: 17784785
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Research group Biologie d'un parasite intracellulaire obligatoire (773)
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PINO, Paco et al. Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii. In: PLOS Pathogens, 2007, vol. 3, n° 8, p. e115. https://archive-ouverte.unige.ch/unige:3969

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Deposited on : 2009-11-05

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