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IgM are associated to Sp alpha (CD5 antigen-like)

Tissot, Jean-Daniel
Vuadens, Françoise
Schifferli, Jürg A
Schneider, Philippe
Duchosal, Michel A
Published in Electrophoresis. 2002, vol. 23, no. 7-8, p. 1203-6
Abstract In 1993, we reported the presence of an IgM-associated peptide (M(r) 44 kDa; pI 5.45) in all immunoglobulin M (IgM) fractions purified from plasma/serum by various methods. This peptide was absent in Ig fractions of non-IgM isotypes. The N-terminal sequence was determined as being APPSGVRLVGGLH. To gain insight into the nature of this peptide, we further analyzed, using modern proteomic tools, the IgM-associated peptide isolated from cryoglobulins. Mass spectrometry revealed three peptides of different masses: 2203.13 (ELGCGAASGTPSGILYEPPAEK), 1564.83 (KPIWLSQMSCSGR), and 1544.77 (EATLQDCPSGPWGK). Theses sequences together with the already known N-terminal sequence allowed us to identity the IgM-associated peptide as Sp alpha (O43866 in TrEMBL database; CD5 antigen-like). Sp alpha is a member of the scavenger receptor cysteine-rich superfamily of proteins. This family includes the T-and B-cell antigens CD5 and CD6, and several of its members influence immune cell fate. Our finding may have important implications in the understanding of the homeostasis of IgM antibodies.
Keywords Amino Acid SequenceAntigens, CD5/metabolismElectrophoresis, Gel, Two-DimensionalImmunoglobulin M/chemistry/metabolismMass SpectrometryMolecular Sequence Data
PMID: 11981870
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Research group Groupe de Protéomique biomédicale (635)
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TISSOT, Jean-Daniel et al. IgM are associated to Sp alpha (CD5 antigen-like). In: Electrophoresis, 2002, vol. 23, n° 7-8, p. 1203-6. https://archive-ouverte.unige.ch/unige:39660

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Deposited on : 2014-08-25

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