en
Scientific article
English

IgM are associated to Sp alpha (CD5 antigen-like)

Published inElectrophoresis, vol. 23, no. 7-8, p. 1203-1206
Publication date2002
Abstract

In 1993, we reported the presence of an IgM-associated peptide (M(r) 44 kDa; pI 5.45) in all immunoglobulin M (IgM) fractions purified from plasma/serum by various methods. This peptide was absent in Ig fractions of non-IgM isotypes. The N-terminal sequence was determined as being APPSGVRLVGGLH. To gain insight into the nature of this peptide, we further analyzed, using modern proteomic tools, the IgM-associated peptide isolated from cryoglobulins. Mass spectrometry revealed three peptides of different masses: 2203.13 (ELGCGAASGTPSGILYEPPAEK), 1564.83 (KPIWLSQMSCSGR), and 1544.77 (EATLQDCPSGPWGK). Theses sequences together with the already known N-terminal sequence allowed us to identity the IgM-associated peptide as Sp alpha (O43866 in TrEMBL database; CD5 antigen-like). Sp alpha is a member of the scavenger receptor cysteine-rich superfamily of proteins. This family includes the T-and B-cell antigens CD5 and CD6, and several of its members influence immune cell fate. Our finding may have important implications in the understanding of the homeostasis of IgM antibodies.

Keywords
  • Amino Acid Sequence
  • Antigens, CD5/metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Immunoglobulin M/chemistry/metabolism
  • Mass Spectrometry
  • Molecular Sequence Data
Citation (ISO format)
TISSOT, Jean-Daniel et al. IgM are associated to Sp alpha (CD5 antigen-like). In: Electrophoresis, 2002, vol. 23, n° 7-8, p. 1203–1206. doi: 10.1002/1522-2683(200204)23:7/8<1203::AID-ELPS1203>3.0.CO;2-1
Main files (1)
Article (Published version)
accessLevelRestricted
ISSN of the journal0173-0835
503views
0downloads

Technical informations

Creation07/14/2014 2:10:00 PM
First validation07/14/2014 2:10:00 PM
Update time03/14/2023 9:32:39 PM
Status update03/14/2023 9:32:39 PM
Last indexation01/16/2024 11:40:01 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack