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Title

Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination

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Published in Journal of Biotechnology. 2006, vol. 122, no. 1, p. 1-4
Abstract Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency of homologous recombination. Here we present an easy method to identify rare mutants, based on PCR analysis of pools of clones. This method also allows the isolation of functional knockout mutants created by a single homologous recombination event, which can be more frequent than a double recombination event.
Keywords AnimalsChromosome Mapping/methodsCloning, Molecular/methodsDNA Mutational Analysis/methodsDictyostelium/geneticsGene Frequency/geneticsGene SilencingGenetic Variation/geneticsMutation/geneticsPolymerase Chain Reaction/methodsRecombination, Genetic/genetics
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PMID: 16198440
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Article (Published version) (131 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Phagocytose (140)
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CHARETTE, Steve, CORNILLON, Sophie Marie, COSSON, Pierre. Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination. In: Journal of Biotechnology, 2006, vol. 122, n° 1, p. 1-4. https://archive-ouverte.unige.ch/unige:39540

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Deposited on : 2014-08-19

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