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Title

Two distinct sensing pathways allow recognition of Klebsiella pneumoniae by Dictyostelium amoebae
Authors
De Lima, Wanessa Cristina
Balestrino, Damien
Forestier, Christiane
Cosson, Pierre
Published in Cellular Microbiology. 2014, vol. 16, no. 3, p. 311-23
Abstract Recognition of bacteria by metazoans is mediated by receptors that recognize different types of microorganisms and elicit specific cellular responses. The soil amoebae Dictyostelium discoideum feeds upon a variable mixture of environmental bacteria, and it is expected to recognize and adapt to various food sources. To date, however, no bacteria-sensing mechanisms have been described. In this study, we isolated a Dictyostelium mutant (fspA KO) unable to grow in the presence of non-capsulated Klebsiella pneumoniae bacteria, but growing as efficiently as wild-type cells in the presence of other bacteria, such as Bacillus subtilis. fspA KO cells were also unable to respond to K. pneumoniae and more specifically to bacterially secreted folate in a chemokinetic assay, while they responded readily to B. subtilis. Remarkably, both WT and fspA KO cells were able to grow in the presence of capsulated LM21 K. pneumoniae, and responded to purified capsule, indicating that capsule recognition may represent an alternative, FspA-independent mechanism for K. pneumoniae sensing. When LM21 capsule synthesis genes were deleted, growth and chemokinetic response were lost for fspA KO cells, but not for WT cells. Altogether, these results indicate that Dictyostelium amoebae use specific recognition mechanisms to respond to different K. pneumoniae elements.
Identifiers
PMID: 24128258
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Article (Published version) (891 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research group Phagocytose (140)
Citation
(ISO format) 		DE LIMA, Wanessa Cristina et al. Two distinct sensing pathways allow recognition of Klebsiella pneumoniae by Dictyostelium amoebae. In: Cellular Microbiology, 2014, vol. 16, n° 3, p. 311-23. https://archive-ouverte.unige.ch/unige:39508

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Deposited on : 2014-08-19

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