UNIGE document Scientific Article
previous document  unige:39258  next document
add to browser collection
Title

Complementary X-ray tomography techniques for histology-validated 3D imaging of soft and hard tissues using plaque-containing blood vessels as examples

Authors
Holme, Margaret N
Schulz, Georg
Deyhle, Hans
Weitkamp, Timm
Beckmann, Felix
Rikhtegar, Farhad
Kurtcuoglu, Vartan
show hidden authors show all authors [1 - 11]
Published in Nature Protocols. 2014, vol. 9, no. 6, p. 1401-15
Abstract A key problem in X-ray computed tomography is choosing photon energies for postmortem specimens containing both soft and hard tissues. Increasing X-ray energy reduces image artifacts from highly absorbing hard tissues including plaque, but it simultaneously decreases contrast in soft tissues including the endothelium. Therefore, identifying the lumen within plaque-containing vessels is challenging. Destructive histology, the gold standard for tissue evaluation, reaches submicron resolution in two dimensions, whereas slice thickness limits spatial resolution in the third. We present a protocol to systematically analyze heterogeneous tissues containing weakly and highly absorbing components in the original wet state, postmortem. Taking the example of atherosclerotic human coronary arteries, the successively acquired 3D data of benchtop and synchrotron radiation-based tomography are validated by histology. The entire protocol requires ∼20 working days, enables differentiation between plaque, muscle and fat tissues without using contrast agents and permits blood flow simulations in vessels with plaque-induced constrictions.
Identifiers
PMID: 24853926
Full text
Article (Published version) (2.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Métastases du foie (657)
Citation
(ISO format)
HOLME, Margaret N et al. Complementary X-ray tomography techniques for histology-validated 3D imaging of soft and hard tissues using plaque-containing blood vessels as examples. In: Nature Protocols, 2014, vol. 9, n° 6, p. 1401-15. https://archive-ouverte.unige.ch/unige:39258

224 hits

0 download

Update

Deposited on : 2014-08-07

Export document
Format :
Citation style :