en
Scientific article
English

Overexpression of focal adhesion kinase in vascular endothelial cells promotes angiogenesis in transgenic mice

Published inCardiovascular research, vol. 64, no. 3, p. 421-430
Publication date2004
Abstract

OBJECTIVE: Focal adhesion kinase is implicated in the regulation of cell adhesion, migration, survival, and cell-cycle progression. However, the functions of focal adhesion kinase in endothelial cell (EC) in vivo remain unclear. This study aims to examine the role of FAK in EC function and angiogenesis in vivo by transgenic mice approach. METHOD: We generated transgenic mice which overexpressed chicken FAK in vascular endothelial cell under the control of the Tie-2 promoter and enhancer. FAK transgene was detected by RT-PCR, immunoprecipitation, and Western blot. The effect of FAK overexpression on angiogenesis was determined using skin wound healing and ischemia skeleton muscle models. RESULTS: Expression of FAK transgene was detected in all vessel-rich tissues. Expression of FAK protein was verified by antibody specific for the exogenous chicken FAK in lung homogenates and isolated EC. In the wound-induced angiogenesis model, the number of vessels in the granulation tissue of healing wound was significantly increased in the transgenic mouse compared to that of wild-type control mice. Similarly, in the ischemia skeleton muscle model, the density of capillaries was significantly increased in the transgenic mouse. CONCLUSION: These results indicate that FAK may play an important role in the promotion of angiogenesis in vivo.

Keywords
  • Animals
  • Capillaries
  • Cell Movement
  • Chickens
  • Endothelial Cells/enzymology
  • Endothelium, Vascular/enzymology
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases
  • Gene Expression
  • Hindlimb
  • Humans
  • Ischemia
  • Mice
  • Mice, Transgenic
  • Models, Animal
  • Muscle, Skeletal/blood supply
  • Neovascularization, Physiologic
  • Protein-Tyrosine Kinases/genetics/metabolism
  • Receptor, TIE-2/genetics
  • Signal Transduction/physiology
  • Wound Healing
NoteComment in: Rizzo MT. Focal adhesion kinase and angiogenesis. Where do we go from here? Cardiovasc Res. 2004 Dec 1;64(3):377-8.Click here to read
Citation (ISO format)
PENG, Xu et al. Overexpression of focal adhesion kinase in vascular endothelial cells promotes angiogenesis in transgenic mice. In: Cardiovascular research, 2004, vol. 64, n° 3, p. 421–430. doi: 10.1016/j.cardiores.2004.07.012
Main files (1)
Article (Accepted version)
accessLevelRestricted
Identifiers
ISSN of the journal0008-6363
496views
0downloads

Technical informations

Creation10/23/2009 2:52:00 PM
First validation10/23/2009 2:52:00 PM
Update time03/14/2023 3:16:55 PM
Status update03/14/2023 3:16:54 PM
Last indexation01/15/2024 7:09:23 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack