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Characterization of novel clonal murine endothelial cell lines with an extended life span

Cavallaro, Ugo
Castelli, Vera
Perilli, Alessandra
Dossi, Romina
Giavazzi, Raffaella
Soria, Marco R.
Published in In vitro cellular & developmental biology. Animal. 2000, vol. 36, no. 5, p. 299-308
Abstract A murine endothelial cell line was recently established from microvessels that had invaded a subcutaneous sponge implant (Dong, Q. G.; Bernasconi, S.; Lostaglio, S., et al. Arterioscl. Thromb. Vasc. Biol. 17:1599-1604; 1997). From these sponge-induced endothelial (SIE) cells, we have isolated two subpopulations endowed with different phenotypic properties. Clone SIE-F consists of large, highly spread cells that have a relatively slow growth rate, form contact-inhibited monolayers, do not grow under anchorage-independent conditions, express elevated levels of thrombospondin-1 (TSP-1) and are not tumorigenic in vivo. In contrast, clone SIE-S2 consists of small, spindle-shaped cells that have a high proliferation rate, do not show contact-inhibition, grow under anchorage-independent conditions, express very low levels of TSP-1 and are tumorigenic in vivo. Both clones express the endothelial markers vascular endothelial-cadherin and vascular intercellular adhesion molecule-1, but do not express CD31 and E-selectin. In addition, SIE-S2 cells, but not SIE-F cells, express the alpha-smooth muscle actin isoform. SIE-S2 cells, but not SIE-F cells, are able to form branching tubes in fibrin gels. The SIE-F and SIE-S2 clones, which have properties of nontransformed and transformed cells, respectively, should provide useful tools to investigate physiological and pathological processes involving vascular endothelium.
Keywords ActinsAnimalsCell CountCell Culture Techniques/methodsCell DivisionCell LineCell SurvivalE-Selectin/biosynthesisEndothelial Growth Factors/pharmacologyEndothelium, Vascular/cytologyFibroblast Growth Factor 2/pharmacologyLymphokines/pharmacologyMiceThrombospondin 1/biosynthesisVascular Cell Adhesion Molecule-1/biosynthesisVascular Endothelial Growth Factor AVascular Endothelial Growth Factors
PMID: 10937833
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CAVALLARO, Ugo et al. Characterization of novel clonal murine endothelial cell lines with an extended life span. In: In Vitro Cellular and Developmental Biology - Animal, 2000, vol. 36, n° 5, p. 299-308. https://archive-ouverte.unige.ch/unige:3878

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Deposited on : 2009-10-23

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