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Scientific article
Open access
English

Characterization of novel clonal murine endothelial cell lines with an extended life span

Published inIn vitro cellular & developmental biology. Animal, vol. 36, no. 5, p. 299-308
Collection
  • Open Access - Licence nationale Springer
Publication date2000
Abstract

A murine endothelial cell line was recently established from microvessels that had invaded a subcutaneous sponge implant (Dong, Q. G.; Bernasconi, S.; Lostaglio, S., et al. Arterioscl. Thromb. Vasc. Biol. 17:1599-1604; 1997). From these sponge-induced endothelial (SIE) cells, we have isolated two subpopulations endowed with different phenotypic properties. Clone SIE-F consists of large, highly spread cells that have a relatively slow growth rate, form contact-inhibited monolayers, do not grow under anchorage-independent conditions, express elevated levels of thrombospondin-1 (TSP-1) and are not tumorigenic in vivo. In contrast, clone SIE-S2 consists of small, spindle-shaped cells that have a high proliferation rate, do not show contact-inhibition, grow under anchorage-independent conditions, express very low levels of TSP-1 and are tumorigenic in vivo. Both clones express the endothelial markers vascular endothelial-cadherin and vascular intercellular adhesion molecule-1, but do not express CD31 and E-selectin. In addition, SIE-S2 cells, but not SIE-F cells, express the alpha-smooth muscle actin isoform. SIE-S2 cells, but not SIE-F cells, are able to form branching tubes in fibrin gels. The SIE-F and SIE-S2 clones, which have properties of nontransformed and transformed cells, respectively, should provide useful tools to investigate physiological and pathological processes involving vascular endothelium.

Keywords
  • Actins
  • Animals
  • Cell Count
  • Cell Culture Techniques/methods
  • Cell Division
  • Cell Line
  • Cell Survival
  • E-Selectin/biosynthesis
  • Endothelial Growth Factors/pharmacology
  • Endothelium, Vascular/cytology
  • Fibroblast Growth Factor 2/pharmacology
  • Lymphokines/pharmacology
  • Mice
  • Thrombospondin 1/biosynthesis
  • Vascular Cell Adhesion Molecule-1/biosynthesis
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
Citation (ISO format)
CAVALLARO, Ugo et al. Characterization of novel clonal murine endothelial cell lines with an extended life span. In: In vitro cellular & developmental biology. Animal, 2000, vol. 36, n° 5, p. 299–308. doi: 10.1290/1071-2690(2000)036<0299:CONCME>2.0.CO;2
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal1071-2690
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186downloads

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