Titles listInhibition of basal p38 or JNK activity enhances epithelial barrier function through differential...
Scientific Article
unige:3875 
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Inhibition of basal p38 or JNK activity enhances epithelial barrier function through differential modulation of claudin expression |
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| Authors | ||
| Published in | American Journal of Physiology. Cell Physiology. 2009, vol. 297, no. 3, p. C775-87 | |
| Abstract | Tight junctions (TJs) form a barrier to the paracellular diffusion of ions and solutes across epithelia. Although transmembrane proteins of the claudin family have emerged as critical determinants of TJ permeability, little is known about the signaling pathways that control their expression. The aim of this study was to assess the role of three mitogen-activated protein kinases (MAPKs), i.e., extracellular signal-regulated kinase-1/2 (ERK1/2), c-Jun NH(2)-terminal kinases (JNKs), and p38 kinases, in the regulation of epithelial barrier function and claudin expression in mammary epithelial cells. Addition of either PD169316 (a p38 inhibitor) or SP600125 (a JNK inhibitor) induced formation of domes (a phenomenon dependent on TJ barrier function) and enhanced transepithelial electrical resistance, whereas U0126 (an inhibitor of the ERK1/2 activators MEK1/MEK2) had no significant effect. Similar results were obtained using mechanistically unrelated p38 or JNK inhibitors. PD169316 increased the expression of claudin-4 and -8, whereas SP600125 increased claudin-4 and -9 and downregulated claudin-8. Silencing of p38alpha by isoform-specific small interfering RNAs increased claudin-4 and -8 mRNAs, whereas silencing of p38beta only increased claudin-4 mRNA. Silencing of either JNK1 or JNK2 increased claudin-9 mRNA expression while decreasing claudin-8 mRNA. Moreover, selective silencing of JNK2 increased claudin-4 and -7 mRNAs. Finally, both PD169316 and SP600125 inhibited the paracellular diffusion of Na(+) and Cl(-) across epithelial monolayers. Collectively, these results provide evidence that inhibition of either p38 or JNK enhances epithelial barrier function by selectively modulating claudin expression, implying that the basal activity of these MAPKs exerts a tonic effect on TJ ionic permeability. | |
| Keywords | Animals — Anthracenes/pharmacology — Butadienes/pharmacology — Cell Line — Dogs — Epithelium/physiology — Female — Imidazoles/pharmacology — MAP Kinase Kinase 4/antagonists & inhibitors/metabolism — Mammary Glands, Animal/cytology — Membrane Proteins/genetics/metabolism — Mice — Nitriles/pharmacology — P38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism | |
| Identifiers | PMID: 19605737 | |
| Full text |
 Article (Author postprint) (1.9 MB) -  Limited access to UNIGE Supplemental data - I (138 Kb) -  Free access Supplemental data - II (123 Kb) - Free access Supplemental data - III (24 Kb) - Free accessOther version: http://ajpcell.physiology.org/cgi/content/full/297/3/C775 |
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| Structures | ||
| Research groups | Groupe Eric Féraille (néphrologie) (29) Etudes et traitement de l'hépatite C et B (554) | |
| Citation (ISO format) | CARROZZINO, Fabio et al. Inhibition of basal p38 or JNK activity enhances epithelial barrier function through differential modulation of claudin expression. In: American Journal of Physiology. Cell Physiology, 2009, vol. 297, n° 3, p. C775-87. https://archive-ouverte.unige.ch/unige:3875 |
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