Scientific article

Bone morphogenetic protein-4 abrogates lumen formation by mammary epithelial cells and promotes invasive growth

ContributorsMontesano, Roberto
Published inBiochemical and biophysical research communications, vol. 353, no. 3, p. 817-822
Publication date2007

Bone morphogenetic proteins (BMPs) are multifunctional cytokines that regulate key developmental processes, but are also overexpressed in many carcinomas. To assess whether BMPs would influence the three-dimensional architecture of epithelial structures, we took advantage of an in vitro model in which mammary epithelial cells form alveolar-like spherical cysts in collagen gels. We found that BMP-4 has a dramatic, biphasic effect on the organization of epithelial cysts. When added in the concentration range of 1-10 ng/ml, the cytokine abrogates lumen formation and induces the outgrowth of multiple invasive cord-like structures. At higher concentrations (20-100 ng/ml), BMP-4 additionally disrupts cell-cell adhesion, resulting in cyst disintegration and scattering of individual cells into the surrounding collagen matrix. The finding that BMP-4 subverts the ability of mammary epithelial cells to form polarized lumen-containing structures and endows them with invasive properties supports the involvement of this cytokine in the progression of breast cancer.

  • Animals
  • Anthracenes/pharmacology
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/pharmacology
  • Butadienes/pharmacology
  • Cell Transformation, Neoplastic/pathology
  • Cells, Cultured
  • Epithelial Cells/drug effects/pathology
  • Female
  • Humans
  • Imidazoles/pharmacology
  • Mammary Glands, Animal/cytology
  • Mice
  • Mitogen-Activated Protein Kinases/antagonists & inhibitors
  • Neoplasm Invasiveness/physiopathology
  • Nitriles/pharmacology
Citation (ISO format)
MONTESANO, Roberto. Bone morphogenetic protein-4 abrogates lumen formation by mammary epithelial cells and promotes invasive growth. In: Biochemical and biophysical research communications, 2007, vol. 353, n° 3, p. 817–822. doi: 10.1016/j.bbrc.2006.12.109
Main files (1)
Article (Accepted version)
ISSN of the journal0006-291X

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