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Scientific article
English

Bone morphogenetic protein-4 abrogates lumen formation by mammary epithelial cells and promotes invasive growth

ContributorsMontesano, Roberto
Published inBiochemical and biophysical research communications, vol. 353, no. 3, p. 817-822
Publication date2007
Abstract

Bone morphogenetic proteins (BMPs) are multifunctional cytokines that regulate key developmental processes, but are also overexpressed in many carcinomas. To assess whether BMPs would influence the three-dimensional architecture of epithelial structures, we took advantage of an in vitro model in which mammary epithelial cells form alveolar-like spherical cysts in collagen gels. We found that BMP-4 has a dramatic, biphasic effect on the organization of epithelial cysts. When added in the concentration range of 1-10 ng/ml, the cytokine abrogates lumen formation and induces the outgrowth of multiple invasive cord-like structures. At higher concentrations (20-100 ng/ml), BMP-4 additionally disrupts cell-cell adhesion, resulting in cyst disintegration and scattering of individual cells into the surrounding collagen matrix. The finding that BMP-4 subverts the ability of mammary epithelial cells to form polarized lumen-containing structures and endows them with invasive properties supports the involvement of this cytokine in the progression of breast cancer.

Keywords
  • Animals
  • Anthracenes/pharmacology
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/pharmacology
  • Butadienes/pharmacology
  • Cell Transformation, Neoplastic/pathology
  • Cells, Cultured
  • Epithelial Cells/drug effects/pathology
  • Female
  • Humans
  • Imidazoles/pharmacology
  • Mammary Glands, Animal/cytology
  • Mice
  • Mitogen-Activated Protein Kinases/antagonists & inhibitors
  • Neoplasm Invasiveness/physiopathology
  • Nitriles/pharmacology
Citation (ISO format)
MONTESANO, Roberto. Bone morphogenetic protein-4 abrogates lumen formation by mammary epithelial cells and promotes invasive growth. In: Biochemical and biophysical research communications, 2007, vol. 353, n° 3, p. 817–822. doi: 10.1016/j.bbrc.2006.12.109
Main files (1)
Article (Accepted version)
accessLevelRestricted
Identifiers
ISSN of the journal0006-291X
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