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Rapid transformation of white adipocytes into fat-oxidizing machines

Cook, William S.
Wang, May-Yun
Park, Byung-Hyun
Unger, Roger H.
Published in Proceedings of the National Academy of Sciences of the United States of America. 2004, vol. 101, no. 7, p. 2058-63
Abstract Adenovirus-induced hyperleptinemia rapidly depletes body fat in normal rats without increasing free fatty acids and ketogenesis, implying that fat-storing adipocytes are oxidizing the fat. To analyze the ultrastructural changes of adipocytes accompanying this functional transformation, we examined the fat tissue by electron microscopy. After 14 days of hyperleptinemia, adipocytes had become shrunken, fatless, and encased in a thick basement-membrane-like matrix. They were crowded with mitochondria that were much smaller than those of brown adipocytes. Their gene expression profile revealed striking up-regulation of peroxisome proliferator-activated receptor gamma coactivator 1alpha (an up-regulator of mitochondrial biogenesis not normally expressed in white fat), increased uncoupling proteins-1 and -2, and down-regulation of lipogenic enzymes. Phosphorylation of both acetyl CoA carboxylase and AMP-activated protein kinase was increased, thus explaining the increase in fatty acid oxidation. The ability to transform adipocytes into unique fat-burning cells may suggest novel therapeutic strategies for obesity.
Keywords Adenoviridae/geneticsAdipocytes/chemistry/enzymology/metabolism/pathologyAdipose Tissue/enzymology/metabolism/pathologyAnimalsBiological MarkersGene Expression RegulationHyperlipidemias/enzymology/genetics/metabolism/pathologyLeptin/genetics/metabolismLipid MetabolismMitochondria/metabolismOxidants/metabolismOxidation-ReductionRNA, Messenger/genetics/metabolismRatsRats, ZuckerTranscription Factors/genetics
PMID: 14769942
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ORCI, Lelio et al. Rapid transformation of white adipocytes into fat-oxidizing machines. In: Proceedings of the National Academy of Sciences of the United States of America, 2004, vol. 101, n° 7, p. 2058-63. doi: 10.1073/pnas.0308258100 https://archive-ouverte.unige.ch/unige:3865

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Deposited on : 2009-10-22

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