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Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells

Wechselberger, Christian
Ebert, Andreas D.
Bianco, Caterina
Khan, Nadia I.
Sun, Youping
Wallace-Jones, Brenda
Salomon, David S.
Published in Experimental Cell Research. 2001, vol. 266, no. 1, p. 95-105
Abstract Cripto-1 is an EGF-CFC protein that performs an important role during early vertebrate development and is overexpressed in several types of human cancer. In the present study mouse EpH4, NMuMG, and TAC-2 mammary epithelial cells that are negative for endogenous cripto-1 expression were transfected with the murine cripto-1 cDNA. Cripto-1-transfected cell lines exhibited functional and physiological differences from the original cell lines including enhanced anchorage-independent growth in soft agar (EpH4 cells), growth in serum-free medium, increased proliferation, and formation of branching, duct-like structures when grown in a three-dimensional collagen type I matrix. Furthermore, cripto-1-expressing cell lines showed elevated migration in vitro in Boyden chamber and wound-healing assays. These results indicate that cripto-1 can function through an autocrine pathway that enables mammary epithelial cells to undergo an epithelial to mesenchymal transition.
Keywords 1-Phosphatidylinositol 3-Kinase/drug effects/metabolismAnimalsBiological Assay/methodsBreast/drug effects/growth & development/metabolismCalcium-Calmodulin-Dependent Protein Kinases/metabolismCell Differentiation/drug effects/physiologyCell Division/drug effects/physiologyCell Movement/drug effects/physiologyCell Size/drug effects/physiologyCells, Cultured/drug effects/metabolismCulture Media, Serum-Free/pharmacologyEpidermal Growth FactorEpithelial Cells/cytology/drug effects/metabolismFemaleGene Expression Regulation, Neoplastic/drug effects/physiologyGlycogen Synthase Kinase 3Membrane GlycoproteinsMiceMice, Inbred BALB CMitogen-Activated Protein Kinases/drug effects/metabolismNeoplasm Metastasis/physiopathologyNeoplasm Proteins/genetics/metabolism/pharmacologyRNA, Messenger/metabolismTransgenes/drug effects/physiology
PMID: 11339828
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WECHSELBERGER, Christian et al. Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells. In: Experimental Cell Research, 2001, vol. 266, n° 1, p. 95-105. https://archive-ouverte.unige.ch/unige:3856

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Deposited on : 2009-10-22

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