en
Scientific article
English

Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells

Published inExperimental cell research, vol. 266, no. 1, p. 95-105
Publication date2001
Abstract

Cripto-1 is an EGF-CFC protein that performs an important role during early vertebrate development and is overexpressed in several types of human cancer. In the present study mouse EpH4, NMuMG, and TAC-2 mammary epithelial cells that are negative for endogenous cripto-1 expression were transfected with the murine cripto-1 cDNA. Cripto-1-transfected cell lines exhibited functional and physiological differences from the original cell lines including enhanced anchorage-independent growth in soft agar (EpH4 cells), growth in serum-free medium, increased proliferation, and formation of branching, duct-like structures when grown in a three-dimensional collagen type I matrix. Furthermore, cripto-1-expressing cell lines showed elevated migration in vitro in Boyden chamber and wound-healing assays. These results indicate that cripto-1 can function through an autocrine pathway that enables mammary epithelial cells to undergo an epithelial to mesenchymal transition.

Keywords
  • 1-Phosphatidylinositol 3-Kinase/drug effects/metabolism
  • Animals
  • Biological Assay/methods
  • Breast/drug effects/growth & development/metabolism
  • Calcium-Calmodulin-Dependent Protein Kinases/metabolism
  • Cell Differentiation/drug effects/physiology
  • Cell Division/drug effects/physiology
  • Cell Movement/drug effects/physiology
  • Cell Size/drug effects/physiology
  • Cells, Cultured/drug effects/metabolism
  • Culture Media, Serum-Free/pharmacology
  • Epidermal Growth Factor
  • Epithelial Cells/cytology/drug effects/metabolism
  • Female
  • Gene Expression Regulation, Neoplastic/drug effects/physiology
  • Glycogen Synthase Kinase 3
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred BALB C
  • Mitogen-Activated Protein Kinases/drug effects/metabolism
  • Neoplasm Metastasis/physiopathology
  • Neoplasm Proteins/genetics/metabolism/pharmacology
  • RNA, Messenger/metabolism
  • Transgenes/drug effects/physiology
Citation (ISO format)
WECHSELBERGER, Christian et al. Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells. In: Experimental cell research, 2001, vol. 266, n° 1, p. 95–105. doi: 10.1006/excr.2001.5195
Main files (1)
Article (Accepted version)
accessLevelRestricted
Identifiers
ISSN of the journal0014-4827
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