UNIGE document Doctoral Thesis
previous document  unige:38415  next document
add to browser collection
Title

The intracellular transport of glycosylphosphatidylinositol-anchored proteins in mammalian Chinese Hamster Ovary cells

Author
Jaensch, Nina
Directors
Defense Thèse de doctorat : Univ. Genève, 2014 - Sc. 4676 - 2014/06/03
Abstract GPI-APs are a class of lipid-anchored proteins expressed on the cell surface of eukaryotes. Their potential interaction with ordered lipid domains enriched in cholesterol and sphingolipids has been proposed to function in their intracellular transport. Here, we examined the importance of two saturated fatty acids within the phosphatidylinositol moiety of GPI-APs. These fatty acids are introduced by the action of lipid remodeling enzymes and required for the GPI-AP association within ordered lipid domains. The fatty acid remodeling is not required for both, efficient Golgi-to-plasma membrane transport and selective endocytosis via CLIC/GEEC pathway, whereas cholesterol depletion significantly affects both pathways independent of their fatty acid structure. Therefore, the mechanism of cholesterol dependence seems not related to the potential interaction with ordered lipid domain mediated by two saturated fatty acids.
Keywords Intracellular protein transportEndocytosisGolgi apparatusGlycosylphosphatidylinositol-anchored proteinsLipid remodelingOrdered lipid domainsLipid raftsClathrin-independent carrierGPI-enriched early endosomal compartmentCholesterolSNAP-tag technology
Identifiers
URN: urn:nbn:ch:unige-384155
Full text
Thesis (8.7 MB) - public document Free access
Structures
Research groups Groupe Gruenberg
Groupe Watanabé
Citation
(ISO format)
JAENSCH, Nina. The intracellular transport of glycosylphosphatidylinositol-anchored proteins in mammalian Chinese Hamster Ovary cells. Université de Genève. Thèse, 2014. https://archive-ouverte.unige.ch/unige:38415

333 hits

306 downloads

Update

Deposited on : 2014-07-02

Export document
Format :
Citation style :