Oriented cell division (OCD) is a mechanism used to generate cellular diversity and to shape tissues. During zebrafish gastrulation divisions in epiblast cells are oriented along the animal-vegetal (A-V) axis. Anthrax toxin receptor (Antxr2a) has a function aligning the mitotic spindle with respect to the A-V axis. Epiblast cells form a cortical F-actin cap, which is aligned with respect to the embryonic axis and therefore with the mitotic spindle. Antxr2a colocalizes with the F-actin cap, forming a cap itself. This mechanism that control OCD is characterized by membrane-cytoskeleton interactions. Lipid composition of the plasma membrane can mediate this interaction. The aim of this work was to identify lipids that are mediating membrane-cytoskeleton interaction. We started by interfering with de novo biosynthetic sphingolipids pathway by knocking down Serine palmitoyltransferase (SPT) enzyme. We found that in Sptlc1 morphants divisions are randomized and that Antxr2a is not polarized forming a cap. With these findings we could conclude that sphingolipids are playing an active role in membrane-cytoskeleton interaction in an OCD context.