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Patient specific stem cell models: focus on glioblastoma and neuronal differentiation aspects of chronic granulomatous disease

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Defense Thèse de doctorat : Univ. Genève, 2014 - Sc. 4657 - 2014/03/31
Abstract Pluripotent stem cells can in vitro replicate key pathophysiological aspects of central nervous system. In the first project, we have developed a 3D co-culture system using cancer stem cells isolated from glioblastoma patients and a human stem cell-derived engineered neural tissue, which allowed us to reproduce numerous hallmarks of glioblastoma in vivo, including invasion and formation of secondary foci. Transcriptomic analysis identified that IFN response genes was induced specifically in the co-culture system that was significantly correlating with patient survival. In a second project, we have focused on the role of ROS generating NOX2 enzyme during neural differentiation. Our study demonstrates that during early stages of neurogenesis there is a regulatory role of NOX2, which is conserved from mouse to human. This suggests a contribution of redox mechanisms in the maintenance of neural stem/progenitor. In conclusion, this study exemplifies the potential of stem cells for the study of human pathophysiology.
Keywords Cancer stem cellsIPSCCNS pathophysiologyNeural differentationNADPH oxidase 2Neural progenitors
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URN: urn:nbn:ch:unige-383307
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Projects Swiss national foundation
3R research foundation
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NAYERNIA, Zeynab. Patient specific stem cell models: focus on glioblastoma and neuronal differentiation aspects of chronic granulomatous disease. Université de Genève. Thèse, 2014. https://archive-ouverte.unige.ch/unige:38330

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Deposited on : 2014-06-30

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