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Title

Translation of pre-spliced RNAs in the nuclear compartment generates peptides for the MHC class I pathway

Authors
Apcher, S.
Millot, G.
Daskalogianni, C.
Manoury, B.
Fahraeus, R.
Published in Proceedings of the National Academy of Sciences. 2013, vol. 110, no. 44, p. 17951-17956
Abstract The scanning of maturing mRNAs by ribosomes plays a key role in the mRNA quality control process. When ribosomes first engage with the newly synthesized mRNA, and if peptides are produced, is unclear, however. Here we show that ribosomal scanning of prespliced mRNAs occurs in the nuclear compartment, and that this event produces peptide substrates for the MHC class I pathway. Inserting antigenic peptide sequences in introns that are spliced out before the mRNAs exit the nuclear compartment results in an equal amount of antigenic peptide products as when the peptides are encoded from the main open reading frame (ORF). Taken together with the detection of intron-encoded nascent peptides and RPS6/RPL7-carrying complexes in the perinucleolar compartment, these results show that peptides are produced by a translation event occurring before mRNA splicing. This suggests that ribosomes occupy and scan mRNAs early in the mRNA maturation process, and suggests a physiological role for nuclear mRNA translation, and also helps explain how the immune system tolerates peptides derived from tissue-specific mRNA splice variants.
Identifiers
PMID: 24082107
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.pnas.org/cgi/doi/10.1073/pnas.1309956110
Structures
Research group Chimie et protéomique clinique (270)
Project FNS: 315230_130830
Citation
(ISO format)
APCHER, S. et al. Translation of pre-spliced RNAs in the nuclear compartment generates peptides for the MHC class I pathway. In: Proceedings of the National Academy of Sciences, 2013, vol. 110, n° 44, p. 17951-17956. https://archive-ouverte.unige.ch/unige:38231

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Deposited on : 2014-06-26

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