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Title

Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

Authors
Tsybin, Yury O.
Hartmer, Ralf
Meyer, Markus
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Published in Journal of the American Society for Mass Spectrometry. 2012, vol. 23, no. 10, p. 1750-1756
Abstract A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whole blood without previous proteolytic cleavage. The analysis was performed in a robust ion trap mass spectrometer operating at nominal mass accuracy and resolution. Subtle differences in globin sequences, resulting with mass shifts of about one Da, can be unambiguously identified. These results suggest that mass spectrometry analysis of entire proteins using electron transfer dissociation can be employed on clinical samples in a workflow compatible with diagnostic applications.
Keywords Electron transfer dissociationSingle reaction monitoringTop-down mass spectrometryHemoglobinClinical proteomics
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PMID: 22869297
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Article (Published version) (497 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://link.springer.com/10.1007/s13361-012-0446-3
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Research group Chimie et protéomique clinique (270)
Citation
(ISO format)
COELHO GRACA, Didia et al. Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples. In: Journal of the American Society for Mass Spectrometry, 2012, vol. 23, n° 10, p. 1750-1756. https://archive-ouverte.unige.ch/unige:38230

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Deposited on : 2014-06-26

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