UNIGE document Scientific Article
previous document  unige:38230  next document
add to browser collection

Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

Tsybin, Yury O.
Hartmer, Ralf
Meyer, Markus
show hidden authors show all authors [1 - 9]
Published in Journal of the American Society for Mass Spectrometry. 2012, vol. 23, no. 10, p. 1750-1756
Collection Open Access - Licence nationale Springer
Abstract A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whole blood without previous proteolytic cleavage. The analysis was performed in a robust ion trap mass spectrometer operating at nominal mass accuracy and resolution. Subtle differences in globin sequences, resulting with mass shifts of about one Da, can be unambiguously identified. These results suggest that mass spectrometry analysis of entire proteins using electron transfer dissociation can be employed on clinical samples in a workflow compatible with diagnostic applications.
Keywords Electron transfer dissociationSingle reaction monitoringTop-down mass spectrometryHemoglobinClinical proteomics
PMID: 22869297
Full text
Research group Chimie et protéomique clinique (270)
(ISO format)
COELHO GRACA, Didia et al. Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples. In: Journal of the American Society for Mass Spectrometry, 2012, vol. 23, n° 10, p. 1750-1756. doi: 10.1007/s13361-012-0446-3 https://archive-ouverte.unige.ch/unige:38230

486 hits



Deposited on : 2014-06-26

Export document
Format :
Citation style :