

Other version: http://www.nature.com/mt/journal/v6/n2/abs/mt2002158a.html
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Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes |
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Authors | ||
Published in | Molecular therapy. 2002, vol. 6, no. 2, p. 199-209 | |
Abstract | Gene therapy is an attractive approach for the treatment of liver disease. We demonstrate that a so-called third-generation human immunodeficiency virus (HIV)-derived vector system can govern the efficient delivery, integration, and stable expression of a transgene into primary human hepatocytes in the complete absence of cell division. We also show that rodent hepatocytes exhibit a significant degree of resistance to HIV vector-mediated transduction, a phenotype that is particularly pronounced in murine hepatocytes and that results from a block in the immediate-early phase of infection. We finally describe a methodology, that allows very high rates of transduction through minimal in vitro manipulation, in which hepatocytes are kept in suspension and reimplanted within a few hours of harvest with a fully preserved engraftment potential. These results have immediate implications for the treatment of liver diseases by the transplantation of genetically modified hepatocytes, an approach that could be applied to a number of hereditary and acquired hepatic disorders. | |
Keywords | Animals — Cell Division — Cell Line — Gene Transfer Techniques — Genetic Therapy/methods — Genetic Vectors — HIV/genetics — HeLa Cells — Hepatocytes/cytology/transplantation — Humans — Lentivirus/genetics — Liver Diseases/therapy — Male — Mice — Rats — Rats, Sprague-Dawley — Transduction, Genetic | |
Identifiers | PMID: 12161186 | |
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![]() ![]() Other version: http://www.nature.com/mt/journal/v6/n2/abs/mt2002158a.html |
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Structures | ||
Research groups | Chirurgie viscérale (104) Chirurgie viscérale (HUG) Hépatologie chirurgicale (327) | |
Citation (ISO format) | NGUYEN, Tuan Huy et al. Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes. In: Molecular therapy, 2002, vol. 6, n° 2, p. 199-209. doi: 10.1006/mthe.2002.0653 https://archive-ouverte.unige.ch/unige:38192 |