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Harmonic nanoparticles for regenerative research

Crotty, Darragh
Prina-Mello, Adriele
Ciepielewski, Daniel
Volkov, Yuri
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Published in Journal of Visualized Experiments. 2014, no. 87
Abstract In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second harmonic generation with no restriction on the excitation wavelength. Multi-photon based methodologies for hESC differentiation into cardiac clusters (maintained as long term air-liquid cultures) are presented in detail. In particular, evidence on how to maximize the intense second harmonic (SH) emission of isolated HNPs during 3D monitoring of beating cardiac tissue in 3D is shown. The analysis of the resulting images to retrieve 3D displacement patterns is also detailed.
PMID: 24836220
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Research group Mécanismes de différentiation cellulaire des cellules cardiaques (536)
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RONZONI, Flavio Lorenzo et al. Harmonic nanoparticles for regenerative research. In: Journal of Visualized Experiments, 2014, n° 87. doi: 10.3791/51333 https://archive-ouverte.unige.ch/unige:38172

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Deposited on : 2014-06-26

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