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Title

Human parainfluenza virus type 2 L protein regions required for interaction with other viral proteins and mRNA capping

Authors
Tsurudome, Masato
Komada, Hiroshi
Ito, Morihiro
Nosaka, Tetsuya
Published in Journal of Virology. 2011, vol. 85, no. 2, p. 725-32
Abstract The large RNA polymerase (L) protein of human parainfluenza virus type 2 (hPIV2) binds the nucleocapsid, phosphoprotein, and V protein, as well as itself, and these interactions are essential for transcription and replication of the viral RNA genome. Although all of these interactions were found to be mediated through the domains within the N terminus of L, the C terminus of the L protein was also required for minigenome reporter gene expression. We have identified a highly conserved rubulavirus domain near the C terminus of the L protein that is required for mRNA synthesis but not for genome replication. Remarkably, this region of L shares homology with a conserved region of cellular capping enzymes that binds GTP and forms a lysyl-GMP enzyme intermediate, the first step in the cellular capping reaction. We propose that this conserved region of L also binds GTP (or GDP) to carry out the second step of the unconventional nonsegmented negative-strand virus capping reaction.
Keywords Cell LineHumansParainfluenza Virus 2, Human/genetics/physiologyProtein BindingRNA Caps/metabolismRNA Processing, Post-TranscriptionalRNA, Messenger/metabolismRNA, Viral/metabolismSequence Homology, Amino AcidTranscription, GeneticViral Proteins/genetics/metabolismVirus Replication
Identifiers
PMID: 21068245
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Article (Published version) (1.8 MB) - public document Free access
Structures
Research group Virologie moléculaire (275)
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NISHIO, Machiko et al. Human parainfluenza virus type 2 L protein regions required for interaction with other viral proteins and mRNA capping. In: Journal of Virology, 2011, vol. 85, n° 2, p. 725-32. https://archive-ouverte.unige.ch/unige:38152

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Deposited on : 2014-06-25

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