UNIGE document Scientific Article
previous document  unige:38151  next document
add to browser collection
Title

Activation of the beta interferon promoter by unnatural Sendai virus infection requires RIG-I and is inhibited by viral C proteins

Authors
Published in Journal of Virology. 2007, vol. 81, no. 22, p. 12227-37
Abstract As infection with wild-type (wt) Sendai virus (SeV) normally activates beta interferon (IFN-beta) very poorly, two unnatural SeV infections were used to study virus-induced IFN-beta activation in mouse embryonic fibroblasts: (i) SeV-DI-H4, which is composed mostly of small, copyback defective interfering (DI) genomes and whose infection overproduces short 5'-triphosphorylated trailer RNAs (pppRNAs) and underproduces viral V and C proteins, and (ii) SeV-GFP(+/-), a coinfection that produces wt amounts of viral gene products but that also produces both green fluorescent protein (GFP) mRNA and its complement, which can form double-stranded RNA (dsRNA) with capped 5' ends. We found that (i) virus-induced signaling to IFN-beta depended predominantly on RIG-I (as opposed to mda-5) for both SeV infections, i.e., that RIG-I senses both pppRNAs and dsRNA without 5'-triphosphorylated ends, and (ii) it is the viral C protein (as opposed to V) that is primarily responsible for countering RIG-I-dependent signaling to IFN-beta. Nondefective SeV that cannot specifically express C proteins not only cannot prevent the effects of transfected poly(I-C) or (ppp)RNAs on IFN-beta activation but also synergistically enhances these effects. SeV-V(minus) infection, in contrast, behaves mostly like wt SeV and counteracts the effects of transfected poly(I-C) or (ppp)RNAs.
Keywords AnimalsCells, CulturedDEAD-box RNA Helicases/genetics/metabolismGene Expression RegulationGreen Fluorescent Proteins/analysis/geneticsInterferon-beta/geneticsMicePromoter Regions, Genetic/geneticsRNA/pharmacologyRNA, Double-Stranded/pharmacologyRNA, Viral/metabolismSendai virus/genetics/metabolismTranscriptional ActivationViral Proteins/genetics/metabolism
Identifiers
PMID: 17804509
Full text
Article (Published version) (454 Kb) - public document Free access
Structures
Research group Virologie moléculaire (275)
Citation
(ISO format)
STRAHLE, Laura Eve et al. Activation of the beta interferon promoter by unnatural Sendai virus infection requires RIG-I and is inhibited by viral C proteins. In: Journal of Virology, 2007, vol. 81, n° 22, p. 12227-37. https://archive-ouverte.unige.ch/unige:38151

321 hits

113 downloads

Update

Deposited on : 2014-06-25

Export document
Format :
Citation style :