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Title

The amino-terminal extensions of the longer Sendai virus C proteins modulate pY701-Stat1 and bulk Stat1 levels independently of interferon signaling

Authors
Goodbourn, Stephen
Published in Journal of Virology. 2003, vol. 77, no. 4, p. 2321-9
Abstract The Sendai virus (SeV) C proteins are known to interact with Stat1 to prevent interferon (IFN)-induced pY701-Stat1 formation and IFN signaling. Nevertheless, pY701-Stat1 levels paradoxically increase during SeV infection. The C proteins also induce bulk Stat1 instability in some cells, similar to rubulavirus V proteins. We have found that SeV infection increases pY701-Stat1 levels even in cells in which bulk Stat1 levels strongly decrease. Remarkably, both the decrease in bulk Stat1 levels and the increase in pY701-Stat1 levels were found to be independent of the IFN signaling system, i.e., these events occur in mutant cells in which various components of the IFN signaling system have been disabled. Consistent with this, the C-induced decrease in Stat1 levels does not require Y701 of Stat1. We present evidence that C interacts with Stat1 in two different ways, one that prevents IFN-induced pY701-Stat1 formation and IFN signaling that has already been documented, and another that induces pY701-Stat1 formation (while decreasing bulk Stat1 levels) in a manner that does not require IFN signaling. These two types of Stat1 interaction are also distinguishable by C gene mutations. In particular, the IFN signaling-independent Stat1 interactions specifically require the amino-terminal extensions of the longer C proteins. The actions of the SeV C proteins in counteracting the cellular antiviral response are clearly more extensive than previously appreciated.
Keywords AnimalsCell LineDNA-Binding Proteins/metabolismHumansInterferons/metabolismMiceSTAT1 Transcription FactorSendai virus/pathogenicitySignal TransductionStructure-Activity RelationshipTrans-Activators/metabolismTransfectionTumor Cells, CulturedViral Proteins/chemistry/genetics/metabolism
Identifiers
PMID: 12551969
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Article (Published version) (1.2 MB) - public document Free access
Structures
Research group Virologie moléculaire (275)
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GARCIN, Dominique et al. The amino-terminal extensions of the longer Sendai virus C proteins modulate pY701-Stat1 and bulk Stat1 levels independently of interferon signaling. In: Journal of Virology, 2003, vol. 77, n° 4, p. 2321-9. https://archive-ouverte.unige.ch/unige:38138

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Deposited on : 2014-06-25

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