Scientific article
Open access

Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis

Published inEMBO journal, vol. 21, no. 19, p. 5141-5150
Publication date2002

Sendai virus (SeV) leader (le) and trailer (tr) RNAs are short transcripts generated during abortive antigenome and genome synthesis, respectively. Recom binant SeV (rSeV) that express tr-like RNAs from the leader region are non-cytopathic and, moreover, prevent wild-type SeV from inducing apoptosis in mixed infections. These rSeV thus appear to have gained a function. Here we report that tr RNA binds to a cellular protein with many links to apoptosis (TIAR) via the AU-rich sequence 5' UUUUAAAUUUU. Duplication of this AU-rich sequence alone within the le RNA confers TIAR binding on this le* RNA and a non-cytopathic phenotype to these rSeV in cell culture. Transgenic overexpression of TIAR during SeV infection promotes apoptosis and reverses the anti-apoptotic effects of le* RNA expression. More over, TIAR overexpression and SeV infection act synergistically to induce apoptosis. These short viral RNAs may act by sequestering TIAR, a multivalent RNA recognition motif (RRM) family RNA-binding protein involved in SeV-induced apoptosis. In this view, tr RNA is not simply a by-product of abortive genome synthesis, but is also an antigenome transcript that modulates the cellular antiviral response.

  • Apoptosis/physiology
  • Cloning, Molecular
  • Cytoplasm/metabolism
  • DNA Damage
  • HeLa Cells
  • Humans
  • Promoter Regions, Genetic
  • RNA, Viral/genetics
  • RNA-Binding Proteins/metabolism
  • Respirovirus Infections/virology
  • Sendai virus/genetics/pathogenicity
  • Transcription, Genetic
Citation (ISO format)
ISENI, Frédéric et al. Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis. In: EMBO journal, 2002, vol. 21, n° 19, p. 5141–5150. doi: 10.1093/emboj/cdf513
Main files (1)
Article (Published version)
ISSN of the journal0261-4189

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