en
Scientific article
Open access
English

Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis

Published inEMBO journal, vol. 21, no. 19, p. 5141-5150
Publication date2002
Abstract

Sendai virus (SeV) leader (le) and trailer (tr) RNAs are short transcripts generated during abortive antigenome and genome synthesis, respectively. Recom binant SeV (rSeV) that express tr-like RNAs from the leader region are non-cytopathic and, moreover, prevent wild-type SeV from inducing apoptosis in mixed infections. These rSeV thus appear to have gained a function. Here we report that tr RNA binds to a cellular protein with many links to apoptosis (TIAR) via the AU-rich sequence 5' UUUUAAAUUUU. Duplication of this AU-rich sequence alone within the le RNA confers TIAR binding on this le* RNA and a non-cytopathic phenotype to these rSeV in cell culture. Transgenic overexpression of TIAR during SeV infection promotes apoptosis and reverses the anti-apoptotic effects of le* RNA expression. More over, TIAR overexpression and SeV infection act synergistically to induce apoptosis. These short viral RNAs may act by sequestering TIAR, a multivalent RNA recognition motif (RRM) family RNA-binding protein involved in SeV-induced apoptosis. In this view, tr RNA is not simply a by-product of abortive genome synthesis, but is also an antigenome transcript that modulates the cellular antiviral response.

Keywords
  • Apoptosis/physiology
  • Cloning, Molecular
  • Cytoplasm/metabolism
  • DNA Damage
  • HeLa Cells
  • Humans
  • Promoter Regions, Genetic
  • RNA, Viral/genetics
  • RNA-Binding Proteins/metabolism
  • Respirovirus Infections/virology
  • Sendai virus/genetics/pathogenicity
  • Transcription, Genetic
Citation (ISO format)
ISENI, Frédéric et al. Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis. In: EMBO journal, 2002, vol. 21, n° 19, p. 5141–5150. doi: 10.1093/emboj/cdf513
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0261-4189
568views
241downloads

Technical informations

Creation06/16/2014 11:58:00 AM
First validation06/16/2014 11:58:00 AM
Update time03/14/2023 9:23:26 PM
Status update03/14/2023 9:23:26 PM
Last indexation01/16/2024 11:09:26 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack