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Title

Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein

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Published in Virology. 1997, vol. 228, no. 1, p. 55-62
Abstract The Sendai virus V protein is a nonstructural trans-frame protein in which a highly conserved cys-rich Zn2+-binding domain is fused to the N-terminal half of the P protein via mRNA editing. Using a recently developed system in which infectious virus is recovered from cDNA, we have engineered a virus in which a translation stop codon was placed at the beginning of the V ORF. Translation of the V(stop) mRNA yields a W-like protein, i.e., a protein composed of the N-terminal half of the P protein alone which is naturally expressed at low levels from the P gene. This V-minus but W-augmented virus was found to replicate normally in cell culture and embryonated chicken eggs. The Sendai virus V protein is thus an accessory protein, and the cys-rich Zn2+-binding domain is likely to function in a specialized role during virus propagation.
Keywords AnimalsCell LineChick EmbryoCricetinaeDogsGene ExpressionHeLa CellsHumansMacaca mulattaOpen Reading FramesRecombinant Fusion Proteins/genetics/isolation & purification/metabolismRespirovirus/genetics/physiologyViral Nonstructural Proteins/genetics/isolation & purification/metabolismVirus Replication
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PMID: 9024809
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Article (Published version) (385 Kb) - public document Free access
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Research group Virologie moléculaire (275)
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DELENDA, Christophe et al. Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein. In: Virology, 1997, vol. 228, n° 1, p. 55-62. https://archive-ouverte.unige.ch/unige:38120

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Deposited on : 2014-06-25

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