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Scientific article
Open access
English

Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein

Published inVirology, vol. 228, no. 1, p. 55-62
Publication date1997
Abstract

The Sendai virus V protein is a nonstructural trans-frame protein in which a highly conserved cys-rich Zn2+-binding domain is fused to the N-terminal half of the P protein via mRNA editing. Using a recently developed system in which infectious virus is recovered from cDNA, we have engineered a virus in which a translation stop codon was placed at the beginning of the V ORF. Translation of the V(stop) mRNA yields a W-like protein, i.e., a protein composed of the N-terminal half of the P protein alone which is naturally expressed at low levels from the P gene. This V-minus but W-augmented virus was found to replicate normally in cell culture and embryonated chicken eggs. The Sendai virus V protein is thus an accessory protein, and the cys-rich Zn2+-binding domain is likely to function in a specialized role during virus propagation.

Keywords
  • Animals
  • Cell Line
  • Chick Embryo
  • Cricetinae
  • Dogs
  • Gene Expression
  • HeLa Cells
  • Humans
  • Macaca mulatta
  • Open Reading Frames
  • Recombinant Fusion Proteins/genetics/isolation & purification/metabolism
  • Respirovirus/genetics/physiology
  • Viral Nonstructural Proteins/genetics/isolation & purification/metabolism
  • Virus Replication
Citation (ISO format)
DELENDA, Christophe et al. Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein. In: Virology, 1997, vol. 228, n° 1, p. 55–62. doi: 10.1006/viro.1996.8354
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Article (Published version)
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ISSN of the journal0042-6822
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