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Capped nonviral sequences at the 5' end of the mRNAs of rice hoja blanca virus RNA4

Ramirez, B C
Calvert, L A
Haenni, A L
Published in Journal of Virology. 1995, vol. 69, no. 3, p. 1951-4
Abstract Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. The RNAs extracted from RHBV-infected tissues contain a population of RNA molecules with 10 to 17 nonviral nucleotides at their 5' end. The RNA-cDNA hybrids resulting from primer extension of such RNA molecules were specifically immunoselected with anti-cap antibodies, indicating that the subgenomic RNAs are capped and probably serve as mRNAs and that the additional nucleotides at their 5' end possibly derive from host mRNAs via a cap-snatching mechanism.
Keywords Base SequenceDNA Primers/chemistryGene Expression Regulation, ViralMolecular Sequence DataOryza sativa/microbiologyPlant Viruses/geneticsRNA CapsRNA Viruses/geneticsRNA, Messenger/geneticsRNA, Viral/genetics
PMID: 7853540
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Research group Virologie moléculaire (275)
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RAMIREZ, B C et al. Capped nonviral sequences at the 5' end of the mRNAs of rice hoja blanca virus RNA4. In: Journal of Virology, 1995, vol. 69, n° 3, p. 1951-4. https://archive-ouverte.unige.ch/unige:37932

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Deposited on : 2014-06-18

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