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Scientific article
English

Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells

Published inEuropean journal of biochemistry, vol. 194, no. 1, p. 279-286
Publication date1990
Abstract

Infection of human epidermoid carcinoma-2 (HEp-2) cells by Herpes simplex virus type 1 (HSV-1) leads to significant activation of inositol phospholipid turnover after 15 min. The effect of neomycin, an inhibitor of inositol phospholipid turnover, has been investigated for its effect on HSV-1 multiplication in HEp-2 cells. HSV-1 multiplication is inhibited by neomycin. This inhibition is not due to a block of virus adsorption or penetration. Neomycin inhibits the expression of virus immediate-early genes, as well as expression of early genes and viral DNA synthesis. In neomycin-treated cells, the usual virion-associated shut off of host protein synthesis does not occur. These results indicate that the inositol phospholipid pathway is involved in immediate-early gene expression and shut off of host protein synthesis in HEp-2 cells.

Keywords
  • Animals
  • Blotting, Northern
  • Carcinoma, Squamous Cell
  • DNA, Viral/biosynthesis
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Viral/drug effects
  • Humans
  • Neomycin/pharmacology
  • Phosphatidylinositols/metabolism
  • RNA, Messenger/genetics
  • RNA, Viral/genetics
  • Simplexvirus/genetics/growth & development
  • Tetradecanoylphorbol Acetate/pharmacology
  • Tumor Cells, Cultured
  • Vero Cells
  • Viral Proteins/biosynthesis/genetics
  • Virus Replication/drug effects
Affiliation Not a UNIGE publication
Citation (ISO format)
GARCIN, Dominique et al. Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells. In: European journal of biochemistry, 1990, vol. 194, n° 1, p. 279–286. doi: 10.1111/j.1432-1033.1990.tb19454.x
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Article (Published version)
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Identifiers
ISSN of the journal0014-2956
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