Scientific Article
previous document  unige:37926  next document
add to browser collection
Title

Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells

Authors
Massé, T
Madjar, J J
Jacquemont, B
Published in European Journal of Biochemistry. 1990, vol. 194, no. 1, p. 279-86
Abstract Infection of human epidermoid carcinoma-2 (HEp-2) cells by Herpes simplex virus type 1 (HSV-1) leads to significant activation of inositol phospholipid turnover after 15 min. The effect of neomycin, an inhibitor of inositol phospholipid turnover, has been investigated for its effect on HSV-1 multiplication in HEp-2 cells. HSV-1 multiplication is inhibited by neomycin. This inhibition is not due to a block of virus adsorption or penetration. Neomycin inhibits the expression of virus immediate-early genes, as well as expression of early genes and viral DNA synthesis. In neomycin-treated cells, the usual virion-associated shut off of host protein synthesis does not occur. These results indicate that the inositol phospholipid pathway is involved in immediate-early gene expression and shut off of host protein synthesis in HEp-2 cells.
Keywords AnimalsBlotting, NorthernCarcinoma, Squamous CellDNA, Viral/biosynthesisDose-Response Relationship, DrugGene Expression Regulation, Viral/drug effectsHumansNeomycin/pharmacologyPhosphatidylinositols/metabolismRNA, Messenger/geneticsRNA, Viral/geneticsSimplexvirus/genetics/growth & developmentTetradecanoylphorbol Acetate/pharmacologyTumor Cells, CulturedVero CellsViral Proteins/biosynthesis/geneticsVirus Replication/drug effects
Identifiers
PMID: 2174777
Full text
Article (Published version) (913 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Citation
(ISO format)
GARCIN, Dominique et al. Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells. In: European Journal of Biochemistry, 1990, vol. 194, n° 1, p. 279-86. https://archive-ouverte.unige.ch/unige:37926

213 hits

0 download

Update

Deposited on : 2014-06-18

Export document
Format :
Citation style :