Bulk optode-based ion selective optical sensors work on the basis of extraction equilibria, and their response toward the analyte ion is known to dependent on the sample pH. This pH dependence has been one of the major disadvantages that have hampered the broad acceptance of bulk optodes in chemical sensing. We present here for the first time the use of exhaustive Ca2+-selective nanosensors that may overcome this pH dependent response. The nanosensors were characterized at different pH and the same linear calibration was obtained in the Ca2+ concentration range from 10–7 M to 10–5 M.