en
Scientific article
English

Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene

Published inVirology, vol. 179, no. 1, p. 208-216
Publication date1990
Abstract

In order to examine cellular gene involvement in HSV-1 expression, we constructed different rat embryo fibroblast cell lines immortalized by adenovirus E1A or c-myc, with or without the human EJ bladder carcinoma transforming oncogene EJ-ras. HSV-1 multiplication was strongly inhibited in cells expressing EJ-ras genes compared to immortalized control cells. Virus adsorption and penetration were not quantitatively modified, but HSV-1 DNA replication was inhibited. The expression of viral thymidine kinase (TK) activity after infection by recombinant virus with the TK coding sequence under immediate-early (IE) promoter control showed that IE gene expression is inhibited in cells expressing EJ-ras. Analysis of IE gene transcription by Northern-blot hybridization and by nuclear run-off transcription assay indicates that this inhibition takes place at the transcriptional level.

Keywords
  • Adenovirus Early Proteins
  • Adenoviruses, Human/genetics
  • Animals
  • Cell Nucleus/metabolism
  • Cell Transformation, Neoplastic
  • Cells, Cultured
  • DNA Replication
  • Embryo, Mammalian
  • Fibroblasts/cytology/enzymology
  • Genes, Viral
  • Genes, ras
  • Humans
  • Nucleic Acid Hybridization
  • Oncogene Proteins, Viral/genetics
  • RNA, Messenger/biosynthesis/genetics
  • Rats
  • Rats, Inbred F344
  • Restriction Mapping
  • Simplexvirus/physiology
  • Thymidine Kinase/metabolism
  • Transcription, Genetic
  • Transfection
  • Vero Cells
  • Virus Replication
Affiliation Not a UNIGE publication
Citation (ISO format)
GARCIN, Dominique et al. Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene. In: Virology, 1990, vol. 179, n° 1, p. 208–216. doi: 10.1016/0042-6822(90)90290-8
Identifiers
ISSN of the journal0042-6822
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