Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene
|Published in||Virology. 1990, vol. 179, no. 1, p. 208-16|
|Abstract||In order to examine cellular gene involvement in HSV-1 expression, we constructed different rat embryo fibroblast cell lines immortalized by adenovirus E1A or c-myc, with or without the human EJ bladder carcinoma transforming oncogene EJ-ras. HSV-1 multiplication was strongly inhibited in cells expressing EJ-ras genes compared to immortalized control cells. Virus adsorption and penetration were not quantitatively modified, but HSV-1 DNA replication was inhibited. The expression of viral thymidine kinase (TK) activity after infection by recombinant virus with the TK coding sequence under immediate-early (IE) promoter control showed that IE gene expression is inhibited in cells expressing EJ-ras. Analysis of IE gene transcription by Northern-blot hybridization and by nuclear run-off transcription assay indicates that this inhibition takes place at the transcriptional level.|
|Keywords||Adenovirus Early Proteins — Adenoviruses, Human/genetics — Animals — Cell Nucleus/metabolism — Cell Transformation, Neoplastic — Cells, Cultured — DNA Replication — Embryo, Mammalian — Fibroblasts/cytology/enzymology — Genes, Viral — Genes, ras — Humans — Nucleic Acid Hybridization — Oncogene Proteins, Viral/genetics — RNA, Messenger/biosynthesis/genetics — Rats — Rats, Inbred F344 — Restriction Mapping — Simplexvirus/physiology — Thymidine Kinase/metabolism — Transcription, Genetic — Transfection — Vero Cells — Virus Replication|
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|GARCIN, Dominique et al. Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene. In: Virology, 1990, vol. 179, n° 1, p. 208-16. https://archive-ouverte.unige.ch/unige:37922|