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Title

Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene

Authors
Michal, Y
Jault, F
Lyon, M
Lenoir, G
Jacquemont, B
Published in Virology. 1990, vol. 179, no. 1, p. 208-16
Abstract In order to examine cellular gene involvement in HSV-1 expression, we constructed different rat embryo fibroblast cell lines immortalized by adenovirus E1A or c-myc, with or without the human EJ bladder carcinoma transforming oncogene EJ-ras. HSV-1 multiplication was strongly inhibited in cells expressing EJ-ras genes compared to immortalized control cells. Virus adsorption and penetration were not quantitatively modified, but HSV-1 DNA replication was inhibited. The expression of viral thymidine kinase (TK) activity after infection by recombinant virus with the TK coding sequence under immediate-early (IE) promoter control showed that IE gene expression is inhibited in cells expressing EJ-ras. Analysis of IE gene transcription by Northern-blot hybridization and by nuclear run-off transcription assay indicates that this inhibition takes place at the transcriptional level.
Keywords Adenovirus Early ProteinsAdenoviruses, Human/geneticsAnimalsCell Nucleus/metabolismCell Transformation, NeoplasticCells, CulturedDNA ReplicationEmbryo, MammalianFibroblasts/cytology/enzymologyGenes, ViralGenes, rasHumansNucleic Acid HybridizationOncogene Proteins, Viral/geneticsRNA, Messenger/biosynthesis/geneticsRatsRats, Inbred F344Restriction MappingSimplexvirus/physiologyThymidine Kinase/metabolismTranscription, GeneticTransfectionVero CellsVirus Replication
Identifiers
PMID: 2171205
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GARCIN, Dominique et al. Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene. In: Virology, 1990, vol. 179, n° 1, p. 208-16. https://archive-ouverte.unige.ch/unige:37922

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Deposited on : 2014-06-18

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