en
Scientific article
English

Thin Layer Coulometry Based on Ion-Exchanger Membranes for Heparin Detection in Undiluted Human Blood

Published inAnalytical chemistry, vol. 86, no. 3, p. 1357-1360
Publication date2014
Abstract

We explore here for the first time a potentially calibration-free methodology for the detection of protamine (and, by titration, heparin) in undiluted human blood in the therapeutic concentration range from 20 to 120 mg L–1. The use of a thin layer sample (5.8 μL) confined between a tubular protamine selective membrane (inner diameter, 600 μm) and a Ag/AgCl wire (diameter 400 μm) achieves an exhaustive depletion from the sample. Coulometry detection was chosen for the interrogation of the thin layer, employing a double pulse technique with 120 s for each pulse. Protamine calibration curves were recorded at physiological concentrations and in undiluted human blood. A linear relationship was obtained in both cases, but a diminished sensitivity was observed in contact with blood, which is explained with a partial passivation of the inner Ag/AgCl element. Heparin–protamine titrations were performed in undiluted human blood samples, mimicking the final application with patients undergoing critical care. The observed values correlate satisfactorily with those of an alternative technique, so-called flash-chronopotentiometry on planar membranes.

Citation (ISO format)
CRESPO PARAVANO, Gaston Adrian et al. Thin Layer Coulometry Based on Ion-Exchanger Membranes for Heparin Detection in Undiluted Human Blood. In: Analytical chemistry, 2014, vol. 86, n° 3, p. 1357–1360. doi: 10.1021/ac403902f
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0003-2700
516views
1downloads

Technical informations

Creation06/17/2014 11:38:00 AM
First validation06/17/2014 11:38:00 AM
Update time03/14/2023 9:21:55 PM
Status update03/14/2023 9:21:55 PM
Last indexation01/16/2024 11:05:01 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack