Scientific article

Thin Layer Coulometry Based on Ion-Exchanger Membranes for Heparin Detection in Undiluted Human Blood

Published inAnalytical chemistry, vol. 86, no. 3, p. 1357-1360
Publication date2014

We explore here for the first time a potentially calibration-free methodology for the detection of protamine (and, by titration, heparin) in undiluted human blood in the therapeutic concentration range from 20 to 120 mg L–1. The use of a thin layer sample (5.8 μL) confined between a tubular protamine selective membrane (inner diameter, 600 μm) and a Ag/AgCl wire (diameter 400 μm) achieves an exhaustive depletion from the sample. Coulometry detection was chosen for the interrogation of the thin layer, employing a double pulse technique with 120 s for each pulse. Protamine calibration curves were recorded at physiological concentrations and in undiluted human blood. A linear relationship was obtained in both cases, but a diminished sensitivity was observed in contact with blood, which is explained with a partial passivation of the inner Ag/AgCl element. Heparin–protamine titrations were performed in undiluted human blood samples, mimicking the final application with patients undergoing critical care. The observed values correlate satisfactorily with those of an alternative technique, so-called flash-chronopotentiometry on planar membranes.

Citation (ISO format)
CRESPO PARAVANO, Gaston Adrian et al. Thin Layer Coulometry Based on Ion-Exchanger Membranes for Heparin Detection in Undiluted Human Blood. In: Analytical chemistry, 2014, vol. 86, n° 3, p. 1357–1360. doi: 10.1021/ac403902f
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Article (Published version)
ISSN of the journal0003-2700

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