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Title

Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study

Authors
Magi, Barbara
Bini, Luca
Perari, Maria Grazia
Fossi, Antonella
Raggiaschi, Roberto
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Published in Electrophoresis. 2002, vol. 23, no. 19, p. 3434-44
Abstract We used two-dimensional (2-D) electrophoresis to analyze the protein composition of fluid recovered by bronchoalveolar lavage (BALF) from patients with sarcoidosis and idiopathic pulmonary fibrosis, two forms of interstitial lung disease with different cellular composition and cytokine profile in BALF. They are also characterized by different pathogenesis and clinical evolution, idiopathic pulmonary fibrosis being less favorable than sarcoidosis due to rapidly progressive pulmonary fibrosis. Thirty-eight proteins or protein fragments, never previously assigned in BALF samples, were identified by various methods including mass fingerprinting of tryptic digests. Comparison of the BALF protein maps of the two groups of patients showed 32 spots with statistically significant disease-related variations in relative abundance. In sarcoidosis we found an increase in the amount of several plasma proteins, while in idiopathic pulmonary fibrosis we observed a statistically significant increase in low-molecular-weight proteins, many of which are involved in inflammatory processes (such as MIF and calgranulin) or antioxidant response (such as antioxidant peroxysomal enzyme and thioredoxin peroxidase 2). 2-D electrophoresis allowed us to identify new BALF proteins and to characterize protein composition in patients with sarcoidosis and idiophatic pulmonary fibrosis. Comparison of the gels of the two diseases showed that they differ in BALF protein profiles as they do in type of immune response.
Keywords AgedBronchoalveolar Lavage Fluid/chemistryElectrophoresis, Gel, Two-DimensionalFemaleHumansMaleMiddle AgedProteins/analysisPulmonary Fibrosis/metabolismSarcoidosis/metabolismSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Identifiers
PMID: 12373774
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Article (Published version) (307 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Groupe de Protéomique biomédicale (635)
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MAGI, Barbara et al. Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study. In: Electrophoresis, 2002, vol. 23, n° 19, p. 3434-44. https://archive-ouverte.unige.ch/unige:37240

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Deposited on : 2014-06-10

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