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Constitutively active UVR8 photoreceptor variant in Arabidopsis

Rizzini, Luca
Van De Slijke, Eveline
Persiau, Geert
Nolf, Jonah
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Published in Proceedings of the National Academy of Sciences of the United States of America. 2013, vol. 110, no. 50, p. 20326-20331
Abstract Arabidopsis thaliana UV RESISTANCE LOCUS 8 (UVR8) is a UV-B photoreceptor that initiates photomorphogenic responses underlying acclimation and UV-B tolerance in plants. UVR8 is a homodimer in its ground state, and UV-B exposure results in its instantaneous monomerization followed by interaction with CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), a major factor in UV-B signaling. UV-B photoreception by UVR8 is based on intrinsic tryptophan aromatic amino acid residues, with tryptophan-285 as the main chromophore. We generated transgenic plants expressing UVR8 with a single amino acid change of tryptophan-285 to alanine. UVR8(W285A) appears monomeric and shows UV-B-independent interaction with COP1. Phenotypically, the plants expressing UVR8(W285A) exhibit constitutive photomorphogenesis associated with constitutive activation of target genes, elevated levels of anthocyanins, and enhanced, acclimation-independent UV-B tolerance. Moreover, we have identified COP1, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 and 2 (RUP1 and RUP2), and the SUPPRESSOR OF PHYA-105 (SPA) family as proteins copurifying with UVR8(W285A). Whereas COP1, RUP1, and RUP2 are known to directly interact with UVR8, we show that SPA1 interacts with UVR8 indirectly through COP1. We conclude that UVR8(W285A) is a constitutively active UVR8 photoreceptor variant in Arabidopsis, as is consistent with the crucial importance of monomer formation and COP1 binding for UVR8 activity.
Keywords Anthocyanins/metabolismArabidopsis/geneticsArabidopsis Proteins/genetics/metabolismCell Cycle Proteins/metabolismChromatography, GelChromatography, LiquidChromosomal Proteins, Non-Histone/geneticsElectrophoresis, Polyacrylamide GelGenetic EngineeringImmunoprecipitationMutation, Missense/geneticsPhenotypePhotoreceptors, Plant/geneticsPlants, Genetically ModifiedReal-Time Polymerase Chain ReactionTandem Mass SpectrometryTwo-Hybrid System TechniquesUbiquitin-Protein Ligases/metabolism
PMID: 24277841
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Research group Groupe Ulm
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HEIJDE, Marc et al. Constitutively active UVR8 photoreceptor variant in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America, 2013, vol. 110, n° 50, p. 20326-20331. doi: 10.1073/pnas.1314336110 https://archive-ouverte.unige.ch/unige:37188

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Deposited on : 2014-06-04

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