en
Scientific article
Open access
English

Individual bioaerosol particle discrimination by multi-photon excited fluorescence

Published inOptics express, vol. 19, no. 24, p. 24516-24521
Publication date2011
Abstract

Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.

Keywords
  • Aerosols
  • Air and pollutants analysis
  • Biological Products
  • Biosensing Techniques
  • Environmental Monitoring
  • Particulate Matter
  • Spectrometry
  • Fluorescence
Citation (ISO format)
KISELEV, Denis, BONACINA, Luigi, WOLF, Jean-Pierre. Individual bioaerosol particle discrimination by multi-photon excited fluorescence. In: Optics express, 2011, vol. 19, n° 24, p. 24516–24521. doi: 10.1364/OE.19.024516
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal1094-4087
589views
2032downloads

Technical informations

Creation06/03/2014 2:01:00 PM
First validation06/03/2014 2:01:00 PM
Update time03/14/2023 9:17:05 PM
Status update03/14/2023 9:17:05 PM
Last indexation01/16/2024 10:48:32 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack