Scientific article
Open access

Individual bioaerosol particle discrimination by multi-photon excited fluorescence

Published inOptics express, vol. 19, no. 24, p. 24516-24521
Publication date2011

Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.

  • Aerosols
  • Air and pollutants analysis
  • Biological Products
  • Biosensing Techniques
  • Environmental Monitoring
  • Particulate Matter
  • Spectrometry
  • Fluorescence
Citation (ISO format)
KISELEV, Denis, BONACINA, Luigi, WOLF, Jean-Pierre. Individual bioaerosol particle discrimination by multi-photon excited fluorescence. In: Optics express, 2011, vol. 19, n° 24, p. 24516–24521. doi: 10.1364/OE.19.024516
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Article (Published version)
ISSN of the journal1094-4087

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