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Doctoral thesis
English

Mechanisms of cellular and intercellular coordination of sodium transport in renal collecting duct principal cells

ContributorsWang, Yubao
Defense date2014-04-15
Abstract

This study was aimed at dissecting mechanisms of coordination between ENaC and Na,K-ATPase as well as between transcellular Na+ transport and paracellular permeability in renal collecting duct principal cells. Aldosterone-clamped rats fed of varying Na+ intake and mCCDcl1 cells that overexpress γ-ENaC in a doxycycline-inducible manner were used. Crosstalk between ENaC and Na,K-ATPase was observed in vivo and in vitro. Increased apical Na+ entry was invariably associated with increased basolateral Na+ extrusion via Na,K-ATPase. This was accounted for by decreased endocytosis and degradation of cell-surface Na,K-ATPase, which was mediated by inhibition of p38 kinase activity. Crosstalk between transcellular Na+ transport and paracellular permeability was also observed in cultured mCCDcl1 cells. Increased transcellular Na+ transport was associated with decreased paracellular permeability as inferred by increased transepithelial resistance. This effect was mediated by decreased β-catenin signaling through the release of its transcriptional suppression of claudin-8 in response to increased transcellular Na+ transport.

eng
Keywords
  • Collecting duct
  • Principal cells
  • ENaC
  • Na
  • K-ATPase
  • Tight junction
  • P38 kinase
  • β-catenin
  • Claudin-8
Citation (ISO format)
WANG, Yubao. Mechanisms of cellular and intercellular coordination of sodium transport in renal collecting duct principal cells. 2014. doi: 10.13097/archive-ouverte/unige:36931
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Creation05/23/2014 11:10:00 AM
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